LOCUS BBa_K2066123 4077 bp DNA linear 27-May-2024 DEFINITION Synthetic Enhancer Project: UNS 55as NRII TetR ACCESSION BBa_K2066123 VERSION BBa_K2066123.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=BBa_K2066541 misc_feature 56..63 /label=Scar promoter 21..55 /label=J23100 misc_feature 84..103 /label=TetR 1 misc_feature 64..83 /label=TetR 1 misc_feature 64..103 /label=R0040 (2x TetR 1) promoter 21..103 /label=BBa_I739101 misc_feature 104..123 /label=BBa_K2066542 misc_feature 124..170 /label=BBa_K2066543 misc_feature 171..258 /label=BBa_K2066532 promoter 259..288 /label=BBa_K2066544 misc_feature 289..310 /label=BBa_K2066545 misc_feature 311..453 /label=BBa_K2066547 CDS 455..1948 /label=BBa_K2066546 misc_feature 1949..2662 /label=BBa_K2066518 stem_loop 2674..2717 /label=stem_loop misc_feature 2663..2742 /label=BBa_B0010 terminator 2663..2742 /label=BBa_B0010 stem_loop 2758..2777 /label=T7 TE misc_feature 2784..2784 /label=stop polyA_site 2778..2791 /label=polya misc_feature 2751..2791 /label=BBa_B0012 terminator 2751..2791 /label=BBa_B0012 terminator 2663..2791 /label=BBa_B0015 misc_feature 2792..2811 /label=BBa_K2066539 misc_feature 2812..2851 /label=BBa_K2066018 promoter 2812..2878 /label=BBa_K2066126 CDS 2879..3928 /label=BBa_K2066538 stem_loop 3940..3983 /label=stem_loop misc_feature 3929..4008 /label=BBa_B0010 terminator 3929..4008 /label=BBa_B0010 stem_loop 4024..4043 /label=T7 TE misc_feature 4050..4050 /label=stop polyA_site 4044..4057 /label=polya misc_feature 4017..4057 /label=BBa_B0012 terminator 4017..4057 /label=BBa_B0012 terminator 3929..4057 /label=BBa_B0015 misc_feature 4058..4077 /label=BBa_K2066540 ORIGIN 1 gctgggagtt cgtagacgga ttgacggcta gctcagtcct aggtacagtg ctagctacta 61 gagtccctat cagtgataga gattccctat cagtgataga gataacaaac gcagaatcca 121 agctcacaat tgcaccaaca tggtgcttaa tgtttccatt gaagcactat attggtgcaa 181 cattgtcttc gacgctagca gcgcgcggag attctagccc cttttcgagg aacgtcccca 241 tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta tcttttttaa 301 ttgtgagcgc tcacaattaa gcttggcgaa ggtggcaccg ggcttggctt atccatcgct 361 cgtaatttga ttgatcagca ttcaggcaaa attgaattta ccagttggcc agggcatacc 421 gagttctcgg tttacctgcc tatcaggaaa taaacgttta tgcaacgagg gatagtctgg 481 gtagtcgatg acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggggcaggt 541 ttaacctgta cgacgtttga gaacggcgca gaagtgctgg aggcgctggc gagcaaaacg 601 ccggatgtgc tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag 661 cagattaaac agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg 721 gatgctgccg tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat 781 atcgacgaag cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag 841 ccgcgtaatg ttcagcttaa cggcccaacg accgatatca tcggcgaagc gccagccatg 901 caggacgtgt tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac 961 ggcgaatccg gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc 1021 gccaaagcgc cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca 1081 gaactgtttg gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt 1141 tttgaacagg ccgatggcgg tacattattc ctcgacgaaa ttggtgatat gccgctggat 1201 gtgcagacgc gtttgctgcg cgtgctggca gacggtcagt tttaccgcgt tggcggctat 1261 gcgccggtga aagtggatgt gcggattatc gctgccactc accagaatct cgaacagcga 1321 gtgcaggaag gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat 1381 ctgccgccgc tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag 1441 gttgccgcgc gcgaactggg cgtagaagcg aagttactgc atccggaaac cgaagctgct 1501 ctgacgcgtc tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta 1561 acggtgatgg ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa 1621 tcaacggttg cggagagtac ttcgcaaatg caaccggaca gctgggcgac gcttcttgcg 1681 cagtgggcag acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca 1741 gagctggagc ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa 1801 gcggcgcggc tactcggctg gggccgcaac accctgacgc gtaagttaaa agagctgggg 1861 atggagtgat tcacagcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt 1921 tacgctttac tgttccagga ggaaaaaaat ggtgagcaag ggcgaagaag ataacatggc 1981 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 2041 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 2101 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 2161 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 2221 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 2281 cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 2341 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 2401 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 2461 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 2521 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 2581 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 2641 ggacgagctg tacaagtaat aaccaggcat caaataaaac gaaaggctca gtcgaaagac 2701 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 2761 tcaccttcgg gtgggccttt ctgcgtttat agcactgaag gtcctcaatc gggtaggccg 2821 gagcaggtga gtcgctctcc aacgtgaagt ttgtcagcta tctgtagccc atctctgcat 2881 ggcaacaggc acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt 2941 gttaatcgat gacaacctgg cgatccatta cgccaaccct gccgcgcaac aactgctcgc 3001 ccaaagctcc cgcaaattgt ttggtacacc gttaccggaa ctgttgagct acttctcatt 3061 aaatatcgag ctgatgcaag aaagtctgga ggcggggcaa ggttttaccg ataacgaagt 3121 gacgctggtc atcgacgggc gctcgcatat cctttctgtg acggcccagc gtatgccgga 3181 cggcatgatc ctgctggaga tggctccgat ggataaccag cgccgcttaa gtcaggaaca 3241 gctacagcac gcccagcagg ttactgcccg tgatttagtg cgcggcctgg cacatgagat 3301 taaaaatccg cttggcggtt tacgtggcgc ggcgcagctg ctcagcaaag cgttacctga 3361 cccatcacta ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaatct 3421 ggtcgaccgt ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa 3481 agtggctgaa cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat 3541 tcgtgattac gatcccagcc taccggaact ggcgcacgac ccggatcaaa ttgaacaggt 3601 cttgctgaat attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat 3661 tctgcgtacc cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc 3721 gcggattgat gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt 3781 ttacccgatg gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgctcgtaa 3841 tttgattgat cagcattcag gcaaaattga atttaccagt tggccagggc ataccgagtt 3901 ctcggtttac ctgcctatca ggaaataacc aggcatcaaa taaaacgaaa ggctcagtcg 3961 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 4021 actggctcac cttcgggtgg gcctttctgc gtttatacac tggaaacatc aaggtcg //