LOCUS BBa_K2066124 4138 bp DNA linear 27-May-2024 DEFINITION Synthetic Enhancer Project: UNS 52s NRII TetR ACCESSION BBa_K2066124 VERSION BBa_K2066124.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=BBa_K2066541 misc_feature 56..63 /label=Scar promoter 21..55 /label=J23100 misc_feature 84..103 /label=TetR 1 misc_feature 64..83 /label=TetR 1 misc_feature 64..103 /label=R0040 (2x TetR 1) promoter 21..103 /label=BBa_I739101 misc_feature 104..123 /label=BBa_K2066542 misc_feature 124..170 /label=BBa_K2066543 misc_feature 171..319 /label=BBa_K2066531 promoter 320..349 /label=BBa_K2066544 misc_feature 350..371 /label=BBa_K2066545 misc_feature 372..514 /label=BBa_K2066547 CDS 516..2009 /label=BBa_K2066546 misc_feature 2010..2723 /label=BBa_K2066518 stem_loop 2735..2778 /label=stem_loop misc_feature 2724..2803 /label=BBa_B0010 terminator 2724..2803 /label=BBa_B0010 stem_loop 2819..2838 /label=T7 TE misc_feature 2845..2845 /label=stop polyA_site 2839..2852 /label=polya misc_feature 2812..2852 /label=BBa_B0012 terminator 2812..2852 /label=BBa_B0012 terminator 2724..2852 /label=BBa_B0015 misc_feature 2853..2872 /label=BBa_K2066539 misc_feature 2873..2912 /label=BBa_K2066018 promoter 2873..2939 /label=BBa_K2066126 CDS 2940..3989 /label=BBa_K2066538 stem_loop 4001..4044 /label=stem_loop misc_feature 3990..4069 /label=BBa_B0010 terminator 3990..4069 /label=BBa_B0010 stem_loop 4085..4104 /label=T7 TE misc_feature 4111..4111 /label=stop polyA_site 4105..4118 /label=polya misc_feature 4078..4118 /label=BBa_B0012 terminator 4078..4118 /label=BBa_B0012 terminator 3990..4118 /label=BBa_B0015 misc_feature 4119..4138 /label=BBa_K2066540 ORIGIN 1 gctgggagtt cgtagacgga ttgacggcta gctcagtcct aggtacagtg ctagctacta 61 gagtccctat cagtgataga gattccctat cagtgataga gataacaaac gcagaatcca 121 agctcacaat tgcaccaaca tggtgcttaa tgtttccatt gaagcactat attggtgcaa 181 cattgtcttc gacgctagct ctctatcact gatagggatg tcggaggctc gcggactcta 241 tcaatgatag agtctcgagt ctggccggat ctctatcact gatagggagc tagcgtcccc 301 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta 361 attgtgagcg ctcacaatta agcttggcga aggtggcacc gggcttggct tatccatcgc 421 tcgtaatttg attgatcagc attcaggcaa aattgaattt accagttggc cagggcatac 481 cgagttctcg gtttacctgc ctatcaggaa ataaacgttt atgcaacgag ggatagtctg 541 ggtagtcgat gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg ctggggcagg 601 tttaacctgt acgacgtttg agaacggcgc agaagtgctg gaggcgctgg cgagcaaaac 661 gccggatgtg ctgctttcag atatccgtat gccgggaatg gacgggctgg cgctgctcaa 721 gcagattaaa cagcgccatc caatgcttcc ggtcatcatt atgaccgcac attccgatct 781 ggatgctgcc gtcagcgcct atcaacaagg ggcgtttgat tatctgccca aaccgtttga 841 tatcgacgaa gcagtggcgc tggttgagcg cgctatcagt cattaccagg aacagcagca 901 gccgcgtaat gttcagctta acggcccaac gaccgatatc atcggcgaag cgccagccat 961 gcaggacgtg ttccgtatta tcggtcggct ttcgcgttct tctattagcg tgctgattaa 1021 cggcgaatcc ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg 1081 cgccaaagcg ccgtttatcg cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc 1141 agaactgttt ggccacgaga aaggcgcgtt tactggcgcg aataccattc gtcaggggcg 1201 ttttgaacag gccgatggcg gtacattatt cctcgacgaa attggtgata tgccgctgga 1261 tgtgcagacg cgtttgctgc gcgtgctggc agacggtcag ttttaccgcg ttggcggcta 1321 tgcgccggtg aaagtggatg tgcggattat cgctgccact caccagaatc tcgaacagcg 1381 agtgcaggaa ggtaagttcc gtgaggatct gttccaccgc ctgaacgtta tccgcgttca 1441 tctgccgccg ctgcgcgaac gtcgggaaga tattccccgt ctggcgcgcc attttttaca 1501 ggttgccgcg cgcgaactgg gcgtagaagc gaagttactg catccggaaa ccgaagctgc 1561 tctgacgcgt ctggcgtggc caggcaacgt gcgccagctg gaaaacacct gccgctggct 1621 aacggtgatg gccgccgggc aggaagtgtt gattcaggat ttgcccggcg aactgtttga 1681 atcaacggtt gcggagagta cttcgcaaat gcaaccggac agctgggcga cgcttcttgc 1741 gcagtgggca gacagagcgc tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc 1801 agagctggag cggacgttac tgacgaccgc gttgcgacat acgcaggggc ataaacagga 1861 agcggcgcgg ctactcggct ggggccgcaa caccctgacg cgtaagttaa aagagctggg 1921 gatggagtga ttcacagctt gtgtgtaaag attgattatt gagcgcaaat tgctggtatt 1981 ttacgcttta ctgttccagg aggaaaaaaa tggtgagcaa gggcgaagaa gataacatgg 2041 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg 2101 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc 2161 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca 2221 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt 2281 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga 2341 ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg 2401 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct 2461 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga 2521 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc 2581 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg 2641 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca 2701 tggacgagct gtacaagtaa taaccaggca tcaaataaaa cgaaaggctc agtcgaaaga 2761 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 2821 ctcaccttcg ggtgggcctt tctgcgttta tagcactgaa ggtcctcaat cgggtaggcc 2881 ggagcaggtg agtcgctctc caacgtgaag tttgtcagct atctgtagcc catctctgca 2941 tggcaacagg cacgcagccc gatgctgggc agatcctcaa ctcgctgatt aacagtattt 3001 tgttaatcga tgacaacctg gcgatccatt acgccaaccc tgccgcgcaa caactgctcg 3061 cccaaagctc ccgcaaattg tttggtacac cgttaccgga actgttgagc tacttctcat 3121 taaatatcga gctgatgcaa gaaagtctgg aggcggggca aggttttacc gataacgaag 3181 tgacgctggt catcgacggg cgctcgcata tcctttctgt gacggcccag cgtatgccgg 3241 acggcatgat cctgctggag atggctccga tggataacca gcgccgctta agtcaggaac 3301 agctacagca cgcccagcag gttactgccc gtgatttagt gcgcggcctg gcacatgaga 3361 ttaaaaatcc gcttggcggt ttacgtggcg cggcgcagct gctcagcaaa gcgttacctg 3421 acccatcact actcgaatat accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatc 3481 tggtcgaccg tctgttgggg ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca 3541 aagtggctga acgcgtggta acgctggtgt cgatggaact gccggacaac gtgcggttga 3601 ttcgtgatta cgatcccagc ctaccggaac tggcgcacga cccggatcaa attgaacagg 3661 tcttgctgaa tattgtgcgc aatgcgctac aggcgctggg gccggaaggc ggtgaaatca 3721 ttctgcgtac ccgcaccgcg tttcaactga ccttacacgg cgagcgctac cggctggcgg 3781 cgcggattga tgtggaagat aacgggccgg gcattccgcc tcatttgcag gatacgctgt 3841 tttacccgat ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc atcgctcgta 3901 atttgattga tcagcattca ggcaaaattg aatttaccag ttggccaggg cataccgagt 3961 tctcggttta cctgcctatc aggaaataac caggcatcaa ataaaacgaa aggctcagtc 4021 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 4081 cactggctca ccttcgggtg ggcctttctg cgtttataca ctggaaacat caaggtcg //