| Types | DnaRegion
|
| Roles | DNA
sequence_feature
|
| Sequences | BBa_K2066501_sequence (Version 1)
|
Description
This is an intermediate part used in the construction of WM16_002. This part contains the BsmBI cut site followed by a single nucleotide spacer (G) followed by sticky end 1. Sticky end 1 is one of three sticky ends used by WM iGEM 2016 for Iterative Capped Assembly. ICA design method based off of Briggs, A. W., Rios, X., Chari, R., Yang, L., Zhang, F., Mali, P., & Church, G. M. (2012). Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers. Nucleic acids research, gks624.
Notes
G was selected as the spacer nucleotide as per Briggs et al., 2012. Sticky End 1 was chosen as per Briggs et al., 2012.
Source
This part was synthesized as part of a gBlock / using IDT DNA synthesis. Part sequence based off of supplemental material from Briggs et al., 2012.
