LOCUS       BBa_K2068001            1427 bp    DNA     linear       01-Jun-2024
DEFINITION  PCQuad Mutant 1
ACCESSION   BBa_K2068001
VERSION     BBa_K2068001.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=T7 Promoter 
     RBS             24..27
                     /label=RBS
     misc_feature    33..38
                     /label=Scar
     misc_feature    39..1427
                     /label=PCQuad Mutant 1
     misc_feature    1404..1421
                     /label=6x His Tag 
     misc_feature    1424..1427
                     /label=Stop Codon
     misc_feature    955..975
                     /label=Thrombin Cleavage Site 
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gccgttcatt accgtaggac
       61 aagaaaattc tacgtctatt gatttgtatt atgaggacca tggtaccggt acgccggtgg
      121 ttctcatcca cggctttccg ctatcaggac attcttggga gcgtcagagc gctgcgcttt
      181 tagatgccgg tgctcgtgta ataacgtacg atagacgcgg ttttggccag agctctcagc
      241 caacgactgg atacgattat gacaccttcg ccgccgattt aaatactgtt ctggaaaccc
      301 tggatcttca ggatgcggtc ttagttggtt ttagtatggg cacaggtgaa gttgcccgct
      361 acgtcagttc ttatggcact gctcgtattg cagcggtagc ttttctggct agcttagaac
      421 cctttctatt aaaaaccgat gataatccgg atggggcggc tccacaggag tttttcgacg
      481 gaattgtggc cgctgtgaaa gccgatagat atgctttcta tactgggttt ttcaatgact
      541 tctataattt agatgaaaac ctgggaacac gcatctcgga agaggctgta cggaactcat
      601 ggaatactgc ggcatctggc ggattctttg ctgccgcagc cgcgccgacc acttggtata
      661 cagattttcg tgcggacatt cctagaattg atgtacctgc cctgattctg cacggtacgg
      721 gcgaccgtac cttaccgatt gagaacactg cccgcgtctt tcataaagct cttccgtccg
      781 ctgagtacgt agaggttgaa ggggcacctc atggtctgtt atggactcat gctgaagaag
      841 tcaacacagc cctgcttgct ttccttgcga aggctcaaga agcccaaaag cagaaattac
      901 tgaccgaagt ggaaacttat gtactatcca tcataccgtc tggtctggtt ccccgcggtt
      961 caggccccct taaagcagaa atcgcacaac gtctggagga tgtcttcgcg ggcaaaaata
     1021 cagacctgga ggtgctgatg gaatggctta agacccgacc gattctgtca ccgttaacga
     1081 agggtatcct aggattcgtt tttaccctga cggtgcccag tgagcgcgga ctgcaacgaa
     1141 gaagattcgt ccaaaacgca ttaaacggga atggtgaccc aaacaatatg gacaaagcgg
     1201 tgaagctgta tcgaaaactt aagcgcgaaa taacattcca tggggccaaa gaaattagcc
     1261 tgagctattc cgctggggcg cttgcttctt gtatgggttt aatatataac cgaatgggtg
     1321 cggtcaccac ggaagtcgcg ttcggattag tatgcgcgac atgcgagcag attgcagact
     1381 ctcagcatag aagccatcgt cagcatcacc accatcacca ttaataa
//