LOCUS       BBa_K2070016             787 bp    DNA     linear       17-May-2024
DEFINITION  Constitutive ecf16 generator
ACCESSION   BBa_K2070016
VERSION     BBa_K2070016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..650
                     /label=BBa_K2070001
     stem_loop       670..713
                     /label=stem_loop
     misc_feature    659..738
                     /label=BBa_B0010
     terminator      659..738
                     /label=BBa_B0010
     stem_loop       754..773
                     /label=T7 TE
     misc_feature    780..780
                     /label=stop
     polyA_site      774..787
                     /label=polya
     misc_feature    747..787
                     /label=BBa_B0012
     terminator      747..787
                     /label=BBa_B0012
     terminator      659..787
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgcagcg caccaacagc caggatgtgc tgagcacccg cgaaagccag cttcaggcgc
      121 tgctgcttca gggcctggcg ggcgatacct ttgcgtatcg ccagtttctg accgcgctgg
      181 cggcgcatat tcgcggcttt ctgcgccgcc gcctgccgca gcatccggcg gaagtggaag
      241 atctgcttca ggaagtgctg ctggcggtgc ataacgcgcg ccatacctat caggcgcgcc
      301 agccgctgac cgcgtgggtg caggcgattg cgcgctataa actggcggat catctgcgca
      361 gccatgcgcg ccgcgaagcg cgccatgatc tgctggatga tgatagcgaa ctgtttgcgg
      421 cgagcgatga acagccggcg caggcgagcc gcgatctggg caaactgctg ggccagctgc
      481 cggatcgcca gcgcctgccg attgtgcatg tgaaactgga aggcctgagc gtggaagaaa
      541 ccgcgcagat taccggcctg agcagcagcg cggtgaaagt gggcattcat cgcggcctga
      601 aagcgctggg caaactgatt cgcggcaaag gccatgatga agatcgctaa tactagagcc
      661 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      721 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      781 gtttata
//