LOCUS       BBa_K2070026            1759 bp    DNA     linear       02-May-2024
DEFINITION  Constitutive ecf16 generator with ecf 16 inducible GFP reporter
ACCESSION   BBa_K2070026
VERSION     BBa_K2070026.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..650
                     /label=BBa_K2070001
     stem_loop       670..713
                     /label=stem_loop
     misc_feature    659..738
                     /label=BBa_B0010
     terminator      659..738
                     /label=BBa_B0010
     stem_loop       754..773
                     /label=T7 TE
     misc_feature    780..780
                     /label=stop
     polyA_site      774..787
                     /label=polya
     misc_feature    747..787
                     /label=BBa_B0012
     terminator      747..787
                     /label=BBa_B0012
     terminator      659..787
                     /label=BBa_B0015
     misc_feature    1..787
                     /label=BBa_K2070016
     promoter        796..875
                     /label=BBa_K2070000
     RBS             884..896
                     /label=RBS-3\Weak
     RBS             890..893
                     /label=RBS
     misc_feature    884..896
                     /label=BBa_B0032
     RBS             884..896
                     /label=BBa_B0032
     CDS             903..1622
                     /label=GFP protein
     CDS             903..1622
                     /label=BBa_E0040
     stem_loop       1642..1685
                     /label=stem_loop
     misc_feature    1631..1710
                     /label=BBa_B0010
     terminator      1631..1710
                     /label=BBa_B0010
     stem_loop       1726..1745
                     /label=T7 TE
     misc_feature    1752..1752
                     /label=stop
     polyA_site      1746..1759
                     /label=polya
     misc_feature    1719..1759
                     /label=BBa_B0012
     terminator      1719..1759
                     /label=BBa_B0012
     misc_feature    884..1759
                     /label=BBa_E0240
     misc_feature    796..1759
                     /label=BBa_K2070006
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgcagcg caccaacagc caggatgtgc tgagcacccg cgaaagccag cttcaggcgc
      121 tgctgcttca gggcctggcg ggcgatacct ttgcgtatcg ccagtttctg accgcgctgg
      181 cggcgcatat tcgcggcttt ctgcgccgcc gcctgccgca gcatccggcg gaagtggaag
      241 atctgcttca ggaagtgctg ctggcggtgc ataacgcgcg ccatacctat caggcgcgcc
      301 agccgctgac cgcgtgggtg caggcgattg cgcgctataa actggcggat catctgcgca
      361 gccatgcgcg ccgcgaagcg cgccatgatc tgctggatga tgatagcgaa ctgtttgcgg
      421 cgagcgatga acagccggcg caggcgagcc gcgatctggg caaactgctg ggccagctgc
      481 cggatcgcca gcgcctgccg attgtgcatg tgaaactgga aggcctgagc gtggaagaaa
      541 ccgcgcagat taccggcctg agcagcagcg cggtgaaagt gggcattcat cgcggcctga
      601 aagcgctggg caaactgatt cgcggcaaag gccatgatga agatcgctaa tactagagcc
      661 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      721 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      781 gtttatatac tagagcttgg atgaaaagaa acccaccgac ggtgtaaccc tggcggccga
      841 tgcaacgaac taactcacag gacgtgctca gcacctacta gagtcacaca ggaaagtact
      901 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      961 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     1021 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     1081 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     1141 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     1201 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     1261 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     1321 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     1381 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1441 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1501 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1561 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1621 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1681 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1741 gggcctttct gcgtttata
//