LOCUS       BBa_K208032              797 bp    DNA     linear       31-May-2024
DEFINITION  Lac pL Promoter/RBS/PhaP/Terminator
ACCESSION   BBa_K208032
VERSION     BBa_K208032.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=Start
     CDS             82..660
                     /label=Phasin
     CDS             82..660
                     /label=BBa_K208001
     stem_loop       680..723
                     /label=stem_loop
     misc_feature    669..748
                     /label=BBa_B0010
     terminator      669..748
                     /label=BBa_B0010
     stem_loop       764..783
                     /label=T7 TE
     misc_feature    790..790
                     /label=stop
     polyA_site      784..797
                     /label=polya
     misc_feature    757..797
                     /label=BBa_B0012
     terminator      757..797
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgatcctc accccggaac aagttgcagc agcgcaaaag
      121 gccaacctcg aaacgctgtt cggcctgacc accaaggcgt ttgaaggcgt cgaaaagctc
      181 gtcgagctga accttcaggt cgtcaagact tcgttcgcag aaggcgttga caacgccaag
      241 aaggcgctgt cggccaagga cgcacaggaa ctgctggcca tccaggccgc agccgtgcag
      301 ccggttgccg aaaagaccct ggcctacacc cgccacctgt atgaaatcgc ttcggaaacc
      361 cagagcgagt tcaccaaggt agccgaggct caactggccg aaggctcgaa gaacgtgcaa
      421 gcgctggtcg agaacctcgc caagaacgcc ccggccggtt cggaatcgac cgtggccatc
      481 gtgaagtcgg cgatctccgc tgccaacaac gcctacgagt cggtgcagaa ggcgaccaag
      541 caagcggtcg aaatcgctga aaccaacttc caggctgcgg ctacggctgc caccaaggct
      601 gcccagcaag ccagcgccac ggcccgtacg gccacggcaa agaagacgac ggctgcctga
      661 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      721 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      781 gcctttctgc gtttata
//