LOCUS       BBa_K208034              942 bp    DNA     linear       01-Jun-2024
DEFINITION  Lac Promoter/RBS/PhaP/Term
ACCESSION   BBa_K208034
VERSION     BBa_K208034.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start
     CDS             227..805
                     /label=Phasin
     CDS             227..805
                     /label=BBa_K208001
     stem_loop       825..868
                     /label=stem_loop
     misc_feature    814..893
                     /label=BBa_B0010
     terminator      814..893
                     /label=BBa_B0010
     stem_loop       909..928
                     /label=T7 TE
     misc_feature    935..935
                     /label=stop
     polyA_site      929..942
                     /label=polya
     misc_feature    902..942
                     /label=BBa_B0012
     terminator      902..942
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga tcctcacccc
      241 ggaacaagtt gcagcagcgc aaaaggccaa cctcgaaacg ctgttcggcc tgaccaccaa
      301 ggcgtttgaa ggcgtcgaaa agctcgtcga gctgaacctt caggtcgtca agacttcgtt
      361 cgcagaaggc gttgacaacg ccaagaaggc gctgtcggcc aaggacgcac aggaactgct
      421 ggccatccag gccgcagccg tgcagccggt tgccgaaaag accctggcct acacccgcca
      481 cctgtatgaa atcgcttcgg aaacccagag cgagttcacc aaggtagccg aggctcaact
      541 ggccgaaggc tcgaagaacg tgcaagcgct ggtcgagaac ctcgccaaga acgccccggc
      601 cggttcggaa tcgaccgtgg ccatcgtgaa gtcggcgatc tccgctgcca acaacgccta
      661 cgagtcggtg cagaaggcga ccaagcaagc ggtcgaaatc gctgaaacca acttccaggc
      721 tgcggctacg gctgccacca aggctgccca gcaagccagc gccacggccc gtacggccac
      781 ggcaaagaag acgacggctg cctgatacta gagccaggca tcaaataaaa cgaaaggctc
      841 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      901 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//