LOCUS       BBa_K208035              856 bp    DNA     linear       13-Jun-2024
DEFINITION  Tet Promoter/RBS/GeneIII/PhaP/Terminator
ACCESSION   BBa_K208035
VERSION     BBa_K208035.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..134
                     /label=Gene III
     misc_feature    81..83
                     /label=Start
     misc_feature    81..134
                     /label=BBa_K208002
     misc_feature    141..143
                     /label=Start
     CDS             141..719
                     /label=Phasin
     CDS             141..719
                     /label=BBa_K208001
     stem_loop       739..782
                     /label=stem_loop
     misc_feature    728..807
                     /label=BBa_B0010
     terminator      728..807
                     /label=BBa_B0010
     stem_loop       823..842
                     /label=T7 TE
     misc_feature    849..849
                     /label=stop
     polyA_site      843..856
                     /label=polya
     misc_feature    816..856
                     /label=BBa_B0012
     terminator      816..856
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaat tattattcgc aattccttta gttgttcctt
      121 tctattctca ctcctactag atgatcctca ccccggaaca agttgcagca gcgcaaaagg
      181 ccaacctcga aacgctgttc ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg
      241 tcgagctgaa ccttcaggtc gtcaagactt cgttcgcaga aggcgttgac aacgccaaga
      301 aggcgctgtc ggccaaggac gcacaggaac tgctggccat ccaggccgca gccgtgcagc
      361 cggttgccga aaagaccctg gcctacaccc gccacctgta tgaaatcgct tcggaaaccc
      421 agagcgagtt caccaaggta gccgaggctc aactggccga aggctcgaag aacgtgcaag
      481 cgctggtcga gaacctcgcc aagaacgccc cggccggttc ggaatcgacc gtggccatcg
      541 tgaagtcggc gatctccgct gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc
      601 aagcggtcga aatcgctgaa accaacttcc aggctgcggc tacggctgcc accaaggctg
      661 cccagcaagc cagcgccacg gcccgtacgg ccacggcaaa gaagacgacg gctgcctgat
      721 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      781 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      841 cctttctgcg tttata
//