LOCUS       BBa_K208038             1002 bp    DNA     linear       08-Jun-2024
DEFINITION  Lac Promoter/RBS/GeneIII/PhaP/Terminator
ACCESSION   BBa_K208038
VERSION     BBa_K208038.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..280
                     /label=Gene III
     misc_feature    227..229
                     /label=Start
     misc_feature    227..280
                     /label=BBa_K208002
     misc_feature    287..289
                     /label=Start
     CDS             287..865
                     /label=Phasin
     CDS             287..865
                     /label=BBa_K208001
     stem_loop       885..928
                     /label=stem_loop
     misc_feature    874..953
                     /label=BBa_B0010
     terminator      874..953
                     /label=BBa_B0010
     stem_loop       969..988
                     /label=T7 TE
     misc_feature    995..995
                     /label=stop
     polyA_site      989..1002
                     /label=polya
     misc_feature    962..1002
                     /label=BBa_B0012
     terminator      962..1002
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaaattatt
      241 attcgcaatt cctttagttg ttcctttcta ttctcactcc tactagatga tcctcacccc
      301 ggaacaagtt gcagcagcgc aaaaggccaa cctcgaaacg ctgttcggcc tgaccaccaa
      361 ggcgtttgaa ggcgtcgaaa agctcgtcga gctgaacctt caggtcgtca agacttcgtt
      421 cgcagaaggc gttgacaacg ccaagaaggc gctgtcggcc aaggacgcac aggaactgct
      481 ggccatccag gccgcagccg tgcagccggt tgccgaaaag accctggcct acacccgcca
      541 cctgtatgaa atcgcttcgg aaacccagag cgagttcacc aaggtagccg aggctcaact
      601 ggccgaaggc tcgaagaacg tgcaagcgct ggtcgagaac ctcgccaaga acgccccggc
      661 cggttcggaa tcgaccgtgg ccatcgtgaa gtcggcgatc tccgctgcca acaacgccta
      721 cgagtcggtg cagaaggcga ccaagcaagc ggtcgaaatc gctgaaacca acttccaggc
      781 tgcggctacg gctgccacca aggctgccca gcaagccagc gccacggccc gtacggccac
      841 ggcaaagaag acgacggctg cctgatacta gagccaggca tcaaataaaa cgaaaggctc
      901 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      961 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//