LOCUS       BBa_K208039              869 bp    DNA     linear       13-Jun-2024
DEFINITION  Lac pL Promoter/RBS/PelB/PhaP/Terminator
ACCESSION   BBa_K208039
VERSION     BBa_K208039.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..147
                     /label=PelB
     misc_feature    82..84
                     /label=Start
     misc_feature    82..147
                     /label=BBa_K208004
     misc_feature    154..156
                     /label=Start
     CDS             154..732
                     /label=Phasin
     CDS             154..732
                     /label=BBa_K208001
     stem_loop       752..795
                     /label=stem_loop
     misc_feature    741..820
                     /label=BBa_B0010
     terminator      741..820
                     /label=BBa_B0010
     stem_loop       836..855
                     /label=T7 TE
     misc_feature    862..862
                     /label=stop
     polyA_site      856..869
                     /label=polya
     misc_feature    829..869
                     /label=BBa_B0012
     terminator      829..869
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaatac ctgctgccga ccgctgctgc tggtctgctg
      121 ctcctcgctg cccagccggc gatggcctac tagatgatcc tcaccccgga acaagttgca
      181 gcagcgcaaa aggccaacct cgaaacgctg ttcggcctga ccaccaaggc gtttgaaggc
      241 gtcgaaaagc tcgtcgagct gaaccttcag gtcgtcaaga cttcgttcgc agaaggcgtt
      301 gacaacgcca agaaggcgct gtcggccaag gacgcacagg aactgctggc catccaggcc
      361 gcagccgtgc agccggttgc cgaaaagacc ctggcctaca cccgccacct gtatgaaatc
      421 gcttcggaaa cccagagcga gttcaccaag gtagccgagg ctcaactggc cgaaggctcg
      481 aagaacgtgc aagcgctggt cgagaacctc gccaagaacg ccccggccgg ttcggaatcg
      541 accgtggcca tcgtgaagtc ggcgatctcc gctgccaaca acgcctacga gtcggtgcag
      601 aaggcgacca agcaagcggt cgaaatcgct gaaaccaact tccaggctgc ggctacggct
      661 gccaccaagg ctgcccagca agccagcgcc acggcccgta cggccacggc aaagaagacg
      721 acggctgcct gatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      781 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      841 accttcgggt gggcctttct gcgtttata
//