LOCUS       BBa_K208041             1014 bp    DNA     linear       13-Jun-2024
DEFINITION  Lac Promoter/RBS/PelB/PhaP/Terminator
ACCESSION   BBa_K208041
VERSION     BBa_K208041.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..292
                     /label=PelB
     misc_feature    227..229
                     /label=Start
     misc_feature    227..292
                     /label=BBa_K208004
     misc_feature    299..301
                     /label=Start
     CDS             299..877
                     /label=Phasin
     CDS             299..877
                     /label=BBa_K208001
     stem_loop       897..940
                     /label=stem_loop
     misc_feature    886..965
                     /label=BBa_B0010
     terminator      886..965
                     /label=BBa_B0010
     stem_loop       981..1000
                     /label=T7 TE
     misc_feature    1007..1007
                     /label=stop
     polyA_site      1001..1014
                     /label=polya
     misc_feature    974..1014
                     /label=BBa_B0012
     terminator      974..1014
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aatacctgct
      241 gccgaccgct gctgctggtc tgctgctcct cgctgcccag ccggcgatgg cctactagat
      301 gatcctcacc ccggaacaag ttgcagcagc gcaaaaggcc aacctcgaaa cgctgttcgg
      361 cctgaccacc aaggcgtttg aaggcgtcga aaagctcgtc gagctgaacc ttcaggtcgt
      421 caagacttcg ttcgcagaag gcgttgacaa cgccaagaag gcgctgtcgg ccaaggacgc
      481 acaggaactg ctggccatcc aggccgcagc cgtgcagccg gttgccgaaa agaccctggc
      541 ctacacccgc cacctgtatg aaatcgcttc ggaaacccag agcgagttca ccaaggtagc
      601 cgaggctcaa ctggccgaag gctcgaagaa cgtgcaagcg ctggtcgaga acctcgccaa
      661 gaacgccccg gccggttcgg aatcgaccgt ggccatcgtg aagtcggcga tctccgctgc
      721 caacaacgcc tacgagtcgg tgcagaaggc gaccaagcaa gcggtcgaaa tcgctgaaac
      781 caacttccag gctgcggcta cggctgccac caaggctgcc cagcaagcca gcgccacggc
      841 ccgtacggcc acggcaaaga agacgacggc tgcctgatac tagagccagg catcaaataa
      901 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      961 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//