LOCUS       BBa_K208042              865 bp    DNA     linear       28-May-2024
DEFINITION  Tet Promoter/RBS/OmpA/PhaP/Terminator
ACCESSION   BBa_K208042
VERSION     BBa_K208042.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..143
                     /label=OmpA
     misc_feature    81..83
                     /label=Start Codon
     misc_feature    81..143
                     /label=BBa_K208003
     misc_feature    150..152
                     /label=Start
     CDS             150..728
                     /label=Phasin
     CDS             150..728
                     /label=BBa_K208001
     stem_loop       748..791
                     /label=stem_loop
     misc_feature    737..816
                     /label=BBa_B0010
     terminator      737..816
                     /label=BBa_B0010
     stem_loop       832..851
                     /label=T7 TE
     misc_feature    858..858
                     /label=stop
     polyA_site      852..865
                     /label=polya
     misc_feature    825..865
                     /label=BBa_B0012
     terminator      825..865
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaga cagctatcgc gattgcagtg gcactggctg
      121 gtttcgctac cgtagcgcag gcctactaga tgatcctcac cccggaacaa gttgcagcag
      181 cgcaaaaggc caacctcgaa acgctgttcg gcctgaccac caaggcgttt gaaggcgtcg
      241 aaaagctcgt cgagctgaac cttcaggtcg tcaagacttc gttcgcagaa ggcgttgaca
      301 acgccaagaa ggcgctgtcg gccaaggacg cacaggaact gctggccatc caggccgcag
      361 ccgtgcagcc ggttgccgaa aagaccctgg cctacacccg ccacctgtat gaaatcgctt
      421 cggaaaccca gagcgagttc accaaggtag ccgaggctca actggccgaa ggctcgaaga
      481 acgtgcaagc gctggtcgag aacctcgcca agaacgcccc ggccggttcg gaatcgaccg
      541 tggccatcgt gaagtcggcg atctccgctg ccaacaacgc ctacgagtcg gtgcagaagg
      601 cgaccaagca agcggtcgaa atcgctgaaa ccaacttcca ggctgcggct acggctgcca
      661 ccaaggctgc ccagcaagcc agcgccacgg cccgtacggc cacggcaaag aagacgacgg
      721 ctgcctgata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      781 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      841 tcgggtgggc ctttctgcgt ttata
//