LOCUS       BBa_K208043              866 bp    DNA     linear       28-May-2024
DEFINITION  Lac pL Promoter/RBS/OmpA/PhaP/Terminator
ACCESSION   BBa_K208043
VERSION     BBa_K208043.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..144
                     /label=OmpA
     misc_feature    82..84
                     /label=Start Codon
     misc_feature    82..144
                     /label=BBa_K208003
     misc_feature    151..153
                     /label=Start
     CDS             151..729
                     /label=Phasin
     CDS             151..729
                     /label=BBa_K208001
     stem_loop       749..792
                     /label=stem_loop
     misc_feature    738..817
                     /label=BBa_B0010
     terminator      738..817
                     /label=BBa_B0010
     stem_loop       833..852
                     /label=T7 TE
     misc_feature    859..859
                     /label=stop
     polyA_site      853..866
                     /label=polya
     misc_feature    826..866
                     /label=BBa_B0012
     terminator      826..866
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaag acagctatcg cgattgcagt ggcactggct
      121 ggtttcgcta ccgtagcgca ggcctactag atgatcctca ccccggaaca agttgcagca
      181 gcgcaaaagg ccaacctcga aacgctgttc ggcctgacca ccaaggcgtt tgaaggcgtc
      241 gaaaagctcg tcgagctgaa ccttcaggtc gtcaagactt cgttcgcaga aggcgttgac
      301 aacgccaaga aggcgctgtc ggccaaggac gcacaggaac tgctggccat ccaggccgca
      361 gccgtgcagc cggttgccga aaagaccctg gcctacaccc gccacctgta tgaaatcgct
      421 tcggaaaccc agagcgagtt caccaaggta gccgaggctc aactggccga aggctcgaag
      481 aacgtgcaag cgctggtcga gaacctcgcc aagaacgccc cggccggttc ggaatcgacc
      541 gtggccatcg tgaagtcggc gatctccgct gccaacaacg cctacgagtc ggtgcagaag
      601 gcgaccaagc aagcggtcga aatcgctgaa accaacttcc aggctgcggc tacggctgcc
      661 accaaggctg cccagcaagc cagcgccacg gcccgtacgg ccacggcaaa gaagacgacg
      721 gctgcctgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      781 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      841 ttcgggtggg cctttctgcg tttata
//