LOCUS       BBa_K208049             1215 bp    DNA     linear       17-May-2024
DEFINITION  Lac Promoter/RBS/TorA/GFP/Term
ACCESSION   BBa_K208049
VERSION     BBa_K208049.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..352
                     /label=TorA
     misc_feature    227..229
                     /label=Start
     misc_feature    227..352
                     /label=BBa_K208005
     misc_feature    359..361
                     /label=Start
     CDS             359..1078
                     /label=GFP Coding Region
     CDS             359..1078
                     /label=BBa_K208000
     stem_loop       1098..1141
                     /label=stem_loop
     misc_feature    1087..1166
                     /label=BBa_B0010
     terminator      1087..1166
                     /label=BBa_B0010
     stem_loop       1182..1201
                     /label=T7 TE
     misc_feature    1208..1208
                     /label=stop
     polyA_site      1202..1215
                     /label=polya
     misc_feature    1175..1215
                     /label=BBa_B0012
     terminator      1175..1215
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acaataacga
      241 tctctttcag gcatcacgtc ggcgttttct ggcacaactc ggcggcttaa ccgtcgccgg
      301 gatgctgggg ccgtcattgt taacgccgcg acgtgcgact gcggcgcaag cgtactagat
      361 ggctagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      421 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg ctacatacgg
      481 aaagcttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      541 tgtcactact ttctcttatg gtgttcaatg cttttcccgt tatccggatc atatgaaacg
      601 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt
      661 caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt
      721 taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc tcggacacaa
      781 actcgagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg
      841 aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggatccgttc aactagcaga
      901 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      961 cctgtcgaca caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct
     1021 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaataata
     1081 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1141 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1201 ctttctgcgt ttata
//