LOCUS       BBa_K2082231            1596 bp    DNA     linear       01-Jun-2024
DEFINITION  RFP under the control of an optimized lacZ promoter combined with 
            the protein SH2:cI434
ACCESSION   BBa_K2082231
VERSION     BBa_K2082231.1  
FEATURES             Location/Qualifiers
     misc_feature    4..17
                     /label=OR1 binding site of cI (434)
     promoter        19..75
                     /label=optimized lacZ promotor
     promoter        1..75
                     /label=BBa_K2082210
     misc_feature    88..91
                     /label=conserved
     RBS             84..95
                     /label=BBa_B0034
     CDS             102..779
                     /label=RFP
     CDS             102..779
                     /label=BBa_K2082291
     misc_feature    788..826
                     /label=B1006
     stem_loop       797..818
                     /label=modified thr terminator
     polyA_site      819..826
                     /label=PolyA
     polyA_site      788..796
                     /label=PolyA
     terminator      788..826
                     /label=BBa_B1006
     misc_feature    1..826
                     /label=BBa_K2082211
     promoter        835..869
                     /label=BBa_J23106
     misc_feature    882..885
                     /label=conserved
     RBS             878..889
                     /label=BBa_B0034
     CDS             896..1180
                     /label=434 cI
     misc_feature    1181..1210
                     /label=cMyc linker
     CDS             1211..1549
                     /label=SH2 domain of Abl1
     CDS             896..1549
                     /label=BBa_K2082207
     misc_feature    1558..1596
                     /label=B1006
     stem_loop       1567..1588
                     /label=modified thr terminator
     polyA_site      1589..1596
                     /label=PolyA
     polyA_site      1558..1566
                     /label=PolyA
     terminator      1558..1596
                     /label=BBa_B1006
     misc_feature    835..1596
                     /label=BBa_K2082225
ORIGIN
        1 aatacaagaa agtttgttca ttaggcaccc cgggctttac tcgtaaagct tccggcgcgt
       61 atgttgtgtc gaccgtacta gagaaagagg agaaatacta gatggcttcc tccgaagacg
      121 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
      181 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
      241 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
      301 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
      361 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
      421 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
      481 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
      541 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
      601 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
      661 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
      721 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
      781 actagagaaa aaaaaacccc gcccctgaca gggcggggtt tttttttact agagtttacg
      841 gctagctcag tcctaggtat agtgctagct actagagaaa gaggagaaat actagatgag
      901 tatttcttcc agggtaaaaa gcaaaaggat tcagcttgga cttaaccagg ctgaacttgc
      961 tcaaaaggtg gggactaccc agcagtctat agagcagctc gaaaacggta aaactaagcg
     1021 accacgcttt ttaccagaac ttgcgtcagc tcttggcgta agtgttgact ggctgctcaa
     1081 tggcacctct gattcgaatg ttagatttgt tgggcacgtt gagcccaaag ggaaatatcc
     1141 attgattagc atggttagag ctggttcgtg gtgtgaagct gaacaaaagc ttatttctga
     1201 agaggacttg agcctggaaa aacatagctg gtatcatggc ccggtgagcc gcaacgcggc
     1261 ggaatatctg ctgagcagcg gcattaacgg cagctttctg gtgcgcgaaa gcgaaagcag
     1321 cccgggccag cgcagcatta gcctgcgcta tgaaggccgc gtgtatcatt atcgcattaa
     1381 caccgcgagc gatggcaaac tgtatgtgag cagcgaaagc cgctttaaca ccctggcgga
     1441 actggtgcat catcatagca ccgtggcgga tggcctgatt accaccctgc attatccggc
     1501 gccgaaacgc aacaaaccga ccgtgtatgg cgtgagcccg aactattaat actagagaaa
     1561 aaaaaacccc gcccctgaca gggcggggtt tttttt
//