LOCUS       BBa_K2088002            2348 bp    DNA     linear       17-May-2024
DEFINITION  dT+mhbR+mhbPt+GFP
ACCESSION   BBa_K2088002
VERSION     BBa_K2088002.1  
FEATURES             Location/Qualifiers
     stem_loop       8..27
                     /label=T7 TE
     misc_feature    34..34
                     /label=stop
     polyA_site      28..41
                     /label=polya
     misc_feature    1..41
                     /label=BBa_B0012
     terminator      1..41
                     /label=BBa_B0012
     stem_loop       62..84
                     /label=stem_loop
     misc_feature    50..95
                     /label=BBa_B0011
     terminator      50..95
                     /label=BBa_B0011
     terminator      1..95
                     /label=BBa_B0014
     misc_feature    102..104
                     /label=ATG start codon
     misc_feature    1017..1019
                     /label=TGA stop codon
     CDS             102..1019
                     /label=MhbR coding region
     CDS             102..1019
                     /label=BBa_K2088011
     promoter        1028..1462
                     /label=Mhbpt
     RBS             1463..1482
                     /label=native RBS
     CDS             1483..2200
                     /label=GFP
     misc_feature    2220..2348
                     /label=T1 terminator
     promoter        1028..2348
                     /label=BBa_K2088004
ORIGIN
        1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
       61 aaagccagat tattaatccg gcttttttat tattttacta gatggcggac tggacgcaga
      121 aagtgaagct gcaccattta cagatgctgg tggcgctggg ggagcaaggc aatctcacgc
      181 aggtggcgaa aatgatgaac attactcagc ccgcgctctc caaatggctc agccagtttg
      241 aagacgaagt cggcatggtt ttgttcgaac gccatagcaa aggcctgcgc ccgaccgaag
      301 gcgggaagct gctgcttcag cacgcccagc ggctgattaa cgatatgtcg cgttcgcagt
      361 atgagatcga gcgttttaag cagggcggac aggtgggcag cctgatgatt ggctgttcgc
      421 cggtggcgac cgactgcgtc tcgcaggcga tcctcgaact gctcaaagag atgccgaccc
      481 tgcacctgaa tatcgaagag aaggtcatga cgccgctgct taacgatctg ctcgccggcg
      541 tggtggatgt ggttgtcgga cgcgtcggcg gacgggcgct ggagctgccg ctgagctacc
      601 gggtgctcta caccgaaccg gtctgctttg tcgcccgtcc cgatcacccg ctggcccaaa
      661 aatcgcggtt aagctgggcc gatctctccc cctggcgctg gatcgtctgg cccaccggca
      721 cgccaattcg cctgagcatc gacaacgcgc tggtggataa cggcgtgatg ctgccggaaa
      781 acaccattga gtccgcctcg atgaacgtca ccagcaacct actgcaaagc agcgatatgg
      841 tctcaattct ctccttgcgt ctggcgcagc gctacgccgc ccagcaccag ctggcgattc
      901 ttaatttgcc gcgcattgaa caaaaaggta gcgttggggt gttctggcga aaaagtgaaa
      961 cgccctcgct ggcgctgagc cggtttctgc actgcctgtc gctgcaatct acgctctgat
     1021 actagaggct cgatctcata ctgcgaacgc gacatatcgt taatcagccg ctgggcgtgc
     1081 tgaagcagca gcttcccgcc ttcggtcggg cgcaggcctt tgctatggcg ttcgaacaaa
     1141 accatgccga cttcgtcttc aaactggctg agccatttgg agagcgcggg ctgagtaatg
     1201 ttcatcattt tcgccacctg cgtgagattg ccttgctccc ccagcgccac cagcatctgt
     1261 aaatggtgca gcttcacttt ctgcgtccag tccgccatta gcgataacct ccaggttatg
     1321 tctatgcccg aaataccatt gtacatttca ttgcctgagt tacaaactcg caacataaaa
     1381 gaaataaata caacacctac ccctacctgc actgaggcat aacgatgact caacgacttg
     1441 aattacagaa gctgctcaac gctactagag gaaagaggag aaatactaga tgcgtaaagg
     1501 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
     1561 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
     1621 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
     1681 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
     1741 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     1801 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     1861 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     1921 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
     1981 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
     2041 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
     2101 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
     2161 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca
     2221 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc cctttcgttt tatctgttgt
     2281 ttgtcggtga acgctctcta ctagagtcac actggctcac ctttcggtgg gcctttctgc
     2341 gtttatat
//