BBa_K2088008 1 BBa_K2088008 catechol 2,3-dioxygenase gene with native rbs 2016-10-07T11:00:00Z 2016-10-08T09:05:18Z Sphingobium sp. YBL2. Sequence found from GenBank: http://www.ncbi.nlm.nih.gov/nuccore/NZ_CP010956 This part includes catechol 2,3-dioxygenase(C23O) gene from Sphingobium sp. YBL2 and its native ribosome binding site. With a promoter before them, this gene can be used to degrade catechol, yeilding bright yellow product 2-hydroxymuconate semialdehyde with an absorbance maximum around 375 nm. As a result, this part can also be utilized as a reporter. false false _2556_ 33751 33751 9 false None false Xia Chen annotation2487916 1 ATG start codon range2487916 1 36 38 annotation2487795 1 C23O coding region range2487795 1 36 968 BBa_K2088009 1 BBa_K2088009 nativeRBS-C23O with double Terminator 2016-10-07T11:00:00Z 2016-10-18T04:16:07Z This is a compostie part including catechol 2,3-dioxygenase gene from Sphingobium sp. YBL2 encoded in part BBa_K2088008. This composite part includes a constitutive promoter (BBa_J23100), a catechol 2,3-dioxygenase gene with native rbs (BBa_K2088008) and the double terminator (BBa_B0015). This device can be used as a reporter, because catechol 2,3-dioxygenase (C23O) can convert catechol to bright yellow product 2-hydroxymuconate semialdehyde. false false _2556_ 33751 33751 9 false Uses J23100, a strong Anderson Promoter, to produce high levels of expression of C23O. false Xia Chen component2516644 1 BBa_K2088008 component2516651 1 BBa_B0015 annotation2516644 1 BBa_K2088008 range2516644 1 1 1021 annotation2516651 1 BBa_B0015 range2516651 1 1030 1158 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2088008_sequence 1 gctgcctgaacaagactgagagaggatttgggacgatgtccatgagaggtgttttgagactgggcgaggtccagatacgcgtgatggacatggcagctgcgcgcgaacattatggcgcaagaatgggcctccacgaggtcatggaagacgaaaagggcctcgtctattataaaccatgggacgatcatgagcaccacagcttcattcttcgcgaggccgattcgccgggcgtcgattatgtagcgttccgcgtctacgatgatgcgacgctgtcgcagcttctgcccaggatcgaggccttcggtctgccggtcatgcatattgccgcagatgtgtacccgaagagtgggcggcgcctccagttcacggcacccagcggccacacgatccagctctatgccgagaaggaactgaccggcaacacgctgggatatcgaaacccgggcgccattccttacgaaggagtggtgcgcggctatcgcgtcaaccggctggaccatgtcctgcttggcggacccaatatcgacgaatgcgcccgcctgttctgcgaggtgttcgacttcgaccttagcgaaaagatcgtcgacgccgagagcgaaatgtcactgtccgtattcctcacctgctcgacgaagccgcacgatatcgcattccagctccaggccgaccccaacaaattccatcatgtatcatttcttttggaatcggtgggcgatctatacaaggcggcggatcttatcgggaaataccgcattccggtcgaagtgccacccaaccgccatggcgtcacgcgcggtgccacgatctacttctttgatccttccggaaaccgcaacgagctcttcgccgaaggctatgtccactatcccgatacgccgactctcgtatgggacaccagcgatcttggccacctaggcttcacccatgacaatcatatgcggcaaagcttcttcgatgtgcttacgtgaccgaaccccgcggtggcggcgtcgccgattggacgcttctaaacctgtggcgt BBa_K2088009_sequence 1 gctgcctgaacaagactgagagaggatttgggacgatgtccatgagaggtgttttgagactgggcgaggtccagatacgcgtgatggacatggcagctgcgcgcgaacattatggcgcaagaatgggcctccacgaggtcatggaagacgaaaagggcctcgtctattataaaccatgggacgatcatgagcaccacagcttcattcttcgcgaggccgattcgccgggcgtcgattatgtagcgttccgcgtctacgatgatgcgacgctgtcgcagcttctgcccaggatcgaggccttcggtctgccggtcatgcatattgccgcagatgtgtacccgaagagtgggcggcgcctccagttcacggcacccagcggccacacgatccagctctatgccgagaaggaactgaccggcaacacgctgggatatcgaaacccgggcgccattccttacgaaggagtggtgcgcggctatcgcgtcaaccggctggaccatgtcctgcttggcggacccaatatcgacgaatgcgcccgcctgttctgcgaggtgttcgacttcgaccttagcgaaaagatcgtcgacgccgagagcgaaatgtcactgtccgtattcctcacctgctcgacgaagccgcacgatatcgcattccagctccaggccgaccccaacaaattccatcatgtatcatttcttttggaatcggtgggcgatctatacaaggcggcggatcttatcgggaaataccgcattccggtcgaagtgccacccaaccgccatggcgtcacgcgcggtgccacgatctacttctttgatccttccggaaaccgcaacgagctcttcgccgaaggctatgtccactatcccgatacgccgactctcgtatgggacaccagcgatcttggccacctaggcttcacccatgacaatcatatgcggcaaagcttcttcgatgtgcttacgtgaccgaaccccgcggtggcggcgtcgccgattggacgcttctaaacctgtggcgttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z