BBa_K2108001 1 BBa_K2108001 YiaT-MCS 2016-10-13T11:00:00Z 2016-10-13T11:11:54Z The truncated YiaT sequence was derived from: Han, M.J., Lee, S.H. (2014). An efficient bacterial surface display system based on a novel outer membrane anchoring element from the Escherichia coli protein YiaT. FEMS Microbiology Letters, 362, 1-7. The E. coli membrane protein YiaT (truncated at R232) and fused with a multiple cloning site. The multiple cloning site is located on the extracellular side of the membrane and is on a long flexible arm of the C-terminal end of YiaT. This system allows for the expression of any protein to the outside of E. coli. false false _2576_ 30893 30893 9 false We ensured that the multiple cloning site did not contain any restrictions sites that were also contained in the prefix or suffix. false Maya de Vos BBa_K2108001_sequence 1 atgcttattaaccgcaacatcgtcgcgcttttcgccctgccattcatggcatcggcgacagcctctgagctttccatcggcgcaggggctgcctataatgagagtccctatcgcggctacaacgagaacaccaaggccattccccttatctcttatgagggggatactttttatgtgcgccagacgactttaggttttatcttatcccaaagtgaaaaaaatgaattaagtttaaccgccagttggatgccgctggaatttgatcctacagacaacgacgactacgcgatgcaacaattagacaaacgtgatagcacggcgatggccggcgtggcttggtaccaccacgagcgttggggtacggtcaaggcgagcgccgccgccgacgttctggacaattctaatggttgggttggggagctgtctgtctttcacaagatgcagattgggcgcttgtcattaaccccggccttgggcgtactttactatgatgagaacttttcggactattattatggaatctcggagtccgaaagtcgccgctcgggcctggcttcatattcggcacaagatgcttgggtgccctatgtaagtttaacggcaaagtatccaatcggcgagcacgtggtcctgatggcgtctgcagggtactccgaattgccggaggagattacggattctccaatgattgaccgtagactacaaagacgacgacgacaaaagactacaaagacgacgacgacaaaagactacaaagacgacgacgacaaaggtaccggcgccctcgagggatccaacgtttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z