LOCUS       BBa_K2109104            1833 bp    DNA     linear       18-May-2024
DEFINITION  Fluorescent Bacterial-2-Hybrid Reporter Cassette
ACCESSION   BBa_K2109104
VERSION     BBa_K2109104.1  
FEATURES             Location/Qualifiers
     misc_feature    1..57
                     /label=ECK120033737
     stem_loop       66..83
                     /label=Lambda CI binding region
     promoter        124..158
                     /label=BBa_J23113
     CDS             221..898
                     /label=mRFP
     misc_feature    934..1062
                     /label=B0015
     CDS             1070..1771
                     /label=mTAG BFP
     RBS             1778..1794
                     /label=B0032
     misc_feature    1803..1833
                     /label=J23101 (strong constitutive promoter)
ORIGIN
        1 ggaaacacag aaaaaagccc gcacctgaca gtgcgggctt tttttttcga ccaaaggtac
       61 tagagtacct ctggcggtga taaggatcca ctgaatgata tgagtagtcc atttagactc
      121 gagctgatgg ctagctcagt cctagggatt atgctagcaa gcttattgtg agcggataac
      181 aattttacta gaggcgtaaa atcaataagg agacaacaag atggcgtcga gcgaagacgt
      241 cattaaagaa tttatgcgct ttaaggtccg catggaaggg tcagtgaacg gtcacgaatt
      301 tgagattgag ggggaggggg agggtcgtcc atacgagggg acccaaacgg caaagctgaa
      361 agtaaccaaa ggaggcccct tgccatttgc ttgggatatc ttatctccac aatttcagta
      421 tggctctaag gcttatgtga aacacccagc agacatccct gattatctta aactttcttt
      481 ccctgaaggc ttcaagtggg agcgtgttat gaattttgag gacggcggcg tggttacggt
      541 gacacaagat tcttcgttac aggacggcga gtttatttac aaagtgaagt tgcgtggcac
      601 aaacttcccg tcggacggtc ccgtcatgca gaagaagact atgggctggg aagcctctac
      661 tgaacgtatg tatcccgaag acggtgcctt aaagggtgaa attaagatgc gtcttaagct
      721 taaggatggc ggccattacg atgctgaagt aaagaccacc tatatggcta aaaagcctgt
      781 tcaattacct ggagcctata agaccgatat caagttagac atcaccagcc acaacgaaga
      841 ttatacaatt gtcgagcaat atgagcgtgc agaaggccgt catagcaccg gcgcataacg
      901 ctgatagtgc tagtgtagat cgctttacta gagtataaac gcagaaaggc ccacccgaag
      961 gtgagccagt gtgactctag tagagagcgt tcaccgacaa acaacagata aaacgaaagg
     1021 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggactagagt taattcagtt
     1081 tatgacccag cttgctaggc aagtcgcaat agcgcgcgac tgcaacctcg tgctgctcca
     1141 cgtaagtctc attattcgct tctttaatgc gttccagacg gtagtcgaca tagtaaacac
     1201 ccggcatctt cagatttttc gccggtttct tgctgcggta cgtcgtcttg atatttgcga
     1261 tcaaatggct gccaccaacc agcttcagtg ccatgtcgtt acggccctcc aggccaccat
     1321 cggccggata cagggtttcc gtaaacgcct cccaacccag ggttttcttc tgcatcaccg
     1381 gaccattgct cgtaaagttg acgccacgaa ttttcacgtt atagatcaga cagccatctt
     1441 gcagggaggt atcttgggtg gcggtcaaca caccaccatc ttcatacgtg gtgacacgct
     1501 cccaggtgaa accttccgga aagctctgtt tgaagaaatc cggaatgccc tgagtatggt
     1561 taatgaacgt tttggaaccg tacaggaagc tggtcgccag aatgtcaaaa gcgaacggca
     1621 gtggaccgcc ctccacaact ttgatacgca tggtttgggt gccttcgtac ggcttaccct
     1681 caccctcaga cgtgcactta aagtggtggt tgtcaacggt gccttccatg tacagcttca
     1741 tgtgcatgtt ctctttgatc agttcgctca tctagtactt tcctgtgtga ctcttactag
     1801 aggcataata cctaggactg agctagctgt aaa
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