BBa_K2110005
1
BBa_K2110005
TPA inducible RFP expression system
2016-10-05T11:00:00Z
2016-10-15T06:00:42Z
BBa_K2110012
BBa_K2110800
BBa_K2110000
PETase gene encodes the PETase enzyme which can degrade PET to terephthalic acid (TPA) and ethylene glycol (EG) with another enzyme MHETase. However, the product TPA can cause inhibition of the reaction and slow down it. This expression system consists of the TPA inducible Leader sequence, the enhanced promoter PGK1(BBa_K2110012), the PETase gene(BBa_K2110800) and Terminator CYC1(BBa_K2110000). It can be induced by product TPA so that this is a positive feedback system. This system is used in Saccharomyces cerevisiae.
false
false
_2578_
32964
32957
9
false
/
false
Le He
component2512251
1
BBa_K2110012
component2512255
1
BBa_K2110000
component2512249
1
BBa_K2110001
component2512254
1
BBa_E1010
annotation2512251
1
BBa_K2110012
range2512251
1
105
885
annotation2512249
1
BBa_K2110001
range2512249
1
1
96
annotation2512255
1
BBa_K2110000
range2512255
1
1606
1853
annotation2512254
1
BBa_E1010
range2512254
1
892
1597
BBa_K2110001
1
BBa_K2110001
TPA inducible Leader sequence (TILS)
2016-10-04T11:00:00Z
2016-10-05T07:09:55Z
It comes from the genome of RhodococcusjostiiRHA1.
This sequence comes from RhodococcusjostiiRHA1. Rhodococcus owns the ability of degrading terephthalic acid (TPA), this pathway is regulated by many genes and some of them can be induced by TPA. The inducing effect is based on a regulatory proteintpaR, which can bind this sequence at the existence of TPA and induce the expression of downstream gene. This sequence should be placed before the sequence of promoter.
false
false
_2578_
32957
32957
9
false
This sequence is very short so if the gel recycling is necessary, the agarose concentration should be increased.
false
Le He
annotation2486062
1
BioBrick
range2486062
1
1
96
BBa_K2110000
1
BBa_K2110000
CYC1 Terminator
2016-10-03T11:00:00Z
2016-10-04T10:55:00Z
It comes from genome sequence of Saccharomyces cerevisiae
CYC1 Terminator is widely used in Saccharomyces cerevisiae. It is a composite part in many vectors of Saccharomyces cerevisiae like plasmid pYES2. Amplifying it by PCR and link it after your protein coding sequence is also feasible if you want to express the gene in Saccharomyces cerevisiae.
false
false
_2578_
32964
32964
9
false
There is no neccessary consideration in detailed design of the sequence
false
Haoran Ding
annotation2485997
1
CYC1 Terminator
range2485997
1
1
1
BBa_E1010
1
mRFP1
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
2004-07-27T11:00:00Z
2015-08-31T04:07:26Z
Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a>
Released HQ 2013
monomeric RFP:
Red Fluorescent Protein.
Excitation peak: 584 nm
Emission peak: 607 nm
false
false
_11_1_
0
52
7
In stock
false
TAATAA double stop codon added (DE).
Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
true
Drew Endy
annotation1014044
1
mrfp1
range1014044
1
1
675
annotation2214014
1
Help:Barcodes
range2214014
1
682
706
BBa_K2110012
1
BBa_K2110012
PGK1 promoter
2016-10-05T11:00:00Z
2016-10-06T12:43:19Z
Saccharomyces cerevisiae
This promoter is a enhanced promoter used in Saccharomyces cerevisiae. It regulates the 3-phosphoglycerate kinase (PGK1) gene in Saccharomyces cerevisiae. This promoter can be used in both plasmid and genome of Saccharomyces cerevisiae.
false
false
_2578_
32957
32957
9
false
/
false
Le He
annotation2486527
1
promoter
range2486527
1
1
781
BBa_K2110012_sequence
1
tattttagattcctgacttcaactcaagacgcacagatattataacatctgcacaataggcatttgcaagaattactcgtgagtaaggaaagagtgaggaactatcgcatacctgcatttaaagatgccgatttgggcgcgaatcctttattttggcttcaccctcatactattatcagggccagaaaaaggaagtgtttccctccttcttgaattgatgttaccctcataaagcacgtggcctcttatcgagaaagaaattaccgtcgctcgtgatttgtttgcaaaaagaacaaaactgaaaaaacccagacacgctcgacttcctgtcttcctattgattgcagcttccaatttcgtcacacaacaaggtcctagcgacggctcacaggttttgtaacaagcaatcgaaggttctggaatggcgggaaagggtttagtaccacatgctatgatgcccactgtgatctccagagcaaagttcgttcgatcgtactgttactctctctctttcaaacagaattgtccgaatcgtgtgacaacaacagcctgttctcacacactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagttttttcaagttcttagatgctttctttttctctttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaaaaca
BBa_K2110005_sequence
1
aatttctcctcagtctggacaaccagtcgggacatcagagaactcgctcggcgggacttgacaagccccgtggctgttcgtataacgaacatatgatactagagtattttagattcctgacttcaactcaagacgcacagatattataacatctgcacaataggcatttgcaagaattactcgtgagtaaggaaagagtgaggaactatcgcatacctgcatttaaagatgccgatttgggcgcgaatcctttattttggcttcaccctcatactattatcagggccagaaaaaggaagtgtttccctccttcttgaattgatgttaccctcataaagcacgtggcctcttatcgagaaagaaattaccgtcgctcgtgatttgtttgcaaaaagaacaaaactgaaaaaacccagacacgctcgacttcctgtcttcctattgattgcagcttccaatttcgtcacacaacaaggtcctagcgacggctcacaggttttgtaacaagcaatcgaaggttctggaatggcgggaaagggtttagtaccacatgctatgatgcccactgtgatctccagagcaaagttcgttcgatcgtactgttactctctctctttcaaacagaattgtccgaatcgtgtgacaacaacagcctgttctcacacactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagttttttcaagttcttagatgctttctttttctctttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaaaacatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagaggcaaattaaagccttcgagcgtcccaaaaccttctcaagcaaggttttcagtataatgttacatgcgtacacgcgtctgtacagaaaaaaaagaaaaatttgaaatataaataacgttcttaatactaacataactataaaaaaataaatagggacctagacttcaggttgtctaactccttccttttcggttagagcggatgtggggggagggcgtgaatgtaagcgtgacataactaattacatga
BBa_E1010_sequence
1
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
BBa_K2110001_sequence
1
aatttctcctcagtctggacaaccagtcgggacatcagagaactcgctcggcgggacttgacaagccccgtggctgttcgtataacgaacatatga
BBa_K2110000_sequence
1
gcaaattaaagccttcgagcgtcccaaaaccttctcaagcaaggttttcagtataatgttacatgcgtacacgcgtctgtacagaaaaaaaagaaaaatttgaaatataaataacgttcttaatactaacataactataaaaaaataaatagggacctagacttcaggttgtctaactccttccttttcggttagagcggatgtggggggagggcgtgaatgtaagcgtgacataactaattacatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z