LOCUS       BBa_K2113003             968 bp    DNA     linear       14-May-2024
DEFINITION  temperature sensitive system with RNA thermometer
ACCESSION   BBa_K2113003
VERSION     BBa_K2113003.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             133..138
                     /label=SD
     misc_feature    117..122
                     /label=Biobrick-Scar-adaptation
     stem_loop       44..58
                     /label=Predicted Stem Loop
     stem_loop       59..79
                     /label=Predicted Stem Loop
     stem_loop       82..114
                     /label=Predicted Stem Loop
     stem_loop       115..139
                     /label=Predicted Stem Loop extending to startcodon
     misc_feature    44..139
                     /label=BBa_K115001
     CDS             146..865
                     /label=GFP protein
     CDS             146..865
                     /label=BBa_E0040
     stem_loop       881..900
                     /label=T7 TE
     misc_feature    907..907
                     /label=stop
     polyA_site      901..914
                     /label=polya
     misc_feature    874..914
                     /label=BBa_B0012
     terminator      874..914
                     /label=BBa_B0012
     stem_loop       935..957
                     /label=stem_loop
     misc_feature    923..968
                     /label=BBa_B0011
     terminator      923..968
                     /label=BBa_B0011
     terminator      874..968
                     /label=BBa_B0014
     terminator      874..968
                     /label=BBa_K823017
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gaggccgcga caagcggtcc
       61 gggcgcccta ggggcccggc ggagacgggc gccggaggtg tccgacgcct gctcgtcaag
      121 ttcttgctcc ttggaggatt actagatgcg taaaggagaa gaacttttca ctggagttgt
      181 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      241 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
      301 actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc
      361 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
      421 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
      481 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
      541 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
      601 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
      661 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      721 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
      781 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
      841 catggatgaa ctatacaaat aataatacta gagtcacact ggctcacctt cgggtgggcc
      901 tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat ccggcttttt
      961 tattattt
//