LOCUS       BBa_K2113006             950 bp    DNA     linear       15-May-2024
DEFINITION  temperature sensitive system with RNA thermometer with cj blue
ACCESSION   BBa_K2113006
VERSION     BBa_K2113006.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             133..138
                     /label=SD
     misc_feature    117..122
                     /label=Biobrick-Scar-adaptation
     stem_loop       44..58
                     /label=Predicted Stem Loop
     stem_loop       59..79
                     /label=Predicted Stem Loop
     stem_loop       82..114
                     /label=Predicted Stem Loop
     stem_loop       115..139
                     /label=Predicted Stem Loop extending to startcodon
     misc_feature    44..139
                     /label=BBa_K115001
     CDS             146..844
                     /label=cjBlue
     CDS             146..847
                     /label=BBa_K592011
     stem_loop       863..882
                     /label=T7 TE
     misc_feature    889..889
                     /label=stop
     polyA_site      883..896
                     /label=polya
     misc_feature    856..896
                     /label=BBa_B0012
     terminator      856..896
                     /label=BBa_B0012
     stem_loop       917..939
                     /label=stem_loop
     misc_feature    905..950
                     /label=BBa_B0011
     terminator      905..950
                     /label=BBa_B0011
     terminator      856..950
                     /label=BBa_B0014
     terminator      856..950
                     /label=BBa_K823017
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gaggccgcga caagcggtcc
       61 gggcgcccta ggggcccggc ggagacgggc gccggaggtg tccgacgcct gctcgtcaag
      121 ttcttgctcc ttggaggatt actagatggc ttccaaaata agcgacaacg tacgtatcaa
      181 actgtatatg gagggcacgg ttaataatca ccacttcatg tgtgaagcgg agggtgaggg
      241 caagccatac gaaggaacgc agatggaaaa cattaaagtg accaaaggag gcccgctgcc
      301 gttctctttt gatatcctga cgccgaactg ccaatatggt tctgtagcca taaccaagta
      361 cacgtcgggg attccggact attttaaaca gtcattccct gaaggtttta cctgggaaag
      421 aaccaccatt tatgaagatg gggcttatct gacaactcag caggaaacca aacttgatgg
      481 aaattgctta gtctacaata ttaaaatcct cggctgcaat tttcccccca atggtcctgt
      541 tatgcagaaa aaaacgcaag gctgggaacc atgttgcgag atgcgctata cacgtgatgg
      601 tgtcttgtgc ggtcagacat taatggcact gaaatgtgcc gatgggaacc atctgacttg
      661 tcatctgcgg actacttacc gatccaaaaa ggcagcgaag gcgttgcaaa tgccaccttt
      721 ccatttttca gaccatcgtc cggaaattgt gaaggttagc gagaacggca cactgtttga
      781 gcagcacgaa agtagtgtgg cacgctattg tcagacatgc ccgagcaaac ttggtcataa
      841 ttaataatac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
      901 agagagagaa tataaaaagc cagattatta atccggcttt tttattattt
//