LOCUS       BBa_K2113009            1000 bp    DNA     linear       14-May-2024
DEFINITION  RNA thermometer and AMP without glycine
ACCESSION   BBa_K2113009
VERSION     BBa_K2113009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             133..138
                     /label=SD
     misc_feature    117..122
                     /label=Biobrick-Scar-adaptation
     stem_loop       44..58
                     /label=Predicted Stem Loop
     stem_loop       59..79
                     /label=Predicted Stem Loop
     stem_loop       82..114
                     /label=Predicted Stem Loop
     stem_loop       115..139
                     /label=Predicted Stem Loop extending to startcodon
     misc_feature    44..139
                     /label=BBa_K115001
     CDS             146..169
                     /label=BBa_K2113004
     misc_feature    182..185
                     /label=conserved
     RBS             178..189
                     /label=BBa_B0034
     CDS             196..894
                     /label=cjBlue
     CDS             196..897
                     /label=BBa_K592011
     stem_loop       913..932
                     /label=T7 TE
     misc_feature    939..939
                     /label=stop
     polyA_site      933..946
                     /label=polya
     misc_feature    906..946
                     /label=BBa_B0012
     terminator      906..946
                     /label=BBa_B0012
     stem_loop       967..989
                     /label=stem_loop
     misc_feature    955..1000
                     /label=BBa_B0011
     terminator      955..1000
                     /label=BBa_B0011
     terminator      906..1000
                     /label=BBa_B0014
     terminator      906..1000
                     /label=BBa_K823017
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gaggccgcga caagcggtcc
       61 gggcgcccta ggggcccggc ggagacgggc gccggaggtg tccgacgcct gctcgtcaag
      121 ttcttgctcc ttggaggatt actagatgtg gcgttggcgt tggcgttaat actagagaaa
      181 gaggagaaat actagatggc ttccaaaata agcgacaacg tacgtatcaa actgtatatg
      241 gagggcacgg ttaataatca ccacttcatg tgtgaagcgg agggtgaggg caagccatac
      301 gaaggaacgc agatggaaaa cattaaagtg accaaaggag gcccgctgcc gttctctttt
      361 gatatcctga cgccgaactg ccaatatggt tctgtagcca taaccaagta cacgtcgggg
      421 attccggact attttaaaca gtcattccct gaaggtttta cctgggaaag aaccaccatt
      481 tatgaagatg gggcttatct gacaactcag caggaaacca aacttgatgg aaattgctta
      541 gtctacaata ttaaaatcct cggctgcaat tttcccccca atggtcctgt tatgcagaaa
      601 aaaacgcaag gctgggaacc atgttgcgag atgcgctata cacgtgatgg tgtcttgtgc
      661 ggtcagacat taatggcact gaaatgtgcc gatgggaacc atctgacttg tcatctgcgg
      721 actacttacc gatccaaaaa ggcagcgaag gcgttgcaaa tgccaccttt ccatttttca
      781 gaccatcgtc cggaaattgt gaaggttagc gagaacggca cactgtttga gcagcacgaa
      841 agtagtgtgg cacgctattg tcagacatgc ccgagcaaac ttggtcataa ttaataatac
      901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa
      961 tataaaaagc cagattatta atccggcttt tttattattt
//