LOCUS       BBa_K2116085             815 bp    DNA     linear       01-Jun-2024
DEFINITION  sfGFP under Tet promoter
ACCESSION   BBa_K2116085
VERSION     BBa_K2116085.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    55..89
                     /label=BBa_K2116093
     misc_feature    90..92
                     /label=ATG start codon
     misc_feature    810..815
                     /label=TAATAA stop codon
     CDS             90..815
                     /label=sfGFP
     CDS             90..815
                     /label=BBa_K2116017
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactctaaa
       61 taaaagcgta taaataagag gaggttatca tgagcaaagg agaagaactt ttcactggag
      121 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtccgtg
      181 gagagggtga aggtgatgct acaaacggaa aactcaccct taaatttatt tgcactactg
      241 gaaaactacc tgttccgtgg ccaacacttg tcactactct gacctatggt gttcaatgct
      301 tttcccgtta tccggatcac atgaaacggc atgacttttt caagagtgcc atgcccgaag
      361 gttatgtaca ggaacgcact atatctttca aagatgacgg gacctacaag acgcgtgctg
      421 aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaagggt attgatttta
      481 aagaagatgg aaacattctt ggacacaaac tcgagtacaa ctttaactca cacaatgtat
      541 acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaacg
      601 ttgaagatgg ttccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
      661 gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgtcctt tcgaaagatc
      721 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
      781 atggcatgga tgagctctac aaaaggcctt aataa
//