LOCUS       BBa_K2120302            2078 bp    DNA     linear       20-May-2024
DEFINITION  araC-pBAD+B0034+RFP+B0015
ACCESSION   BBa_K2120302
VERSION     BBa_K2120302.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     misc_feature    909..924
                     /label=AraO
     misc_feature    1066..1077
                     /label=AraO1
     promoter        1153..1181
                     /label=AraPromoter
     promoter        1..1209
                     /label=BBa_K808000
     misc_feature    1222..1225
                     /label=conserved
     RBS             1218..1229
                     /label=BBa_B0034
     CDS             1236..1910
                     /label=mrfp1
     misc_feature    1917..1941
                     /label=Help:Barcodes
     CDS             1236..1941
                     /label=BBa_E1010
     stem_loop       1961..2004
                     /label=stem_loop
     misc_feature    1950..2029
                     /label=BBa_B0010
     terminator      1950..2029
                     /label=BBa_B0010
     stem_loop       2045..2064
                     /label=T7 TE
     misc_feature    2071..2071
                     /label=stop
     polyA_site      2065..2078
                     /label=polya
     misc_feature    2038..2078
                     /label=BBa_B0012
     terminator      2038..2078
                     /label=BBa_B0012
     terminator      1950..2078
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 tgggctaact actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
     1261 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
     1321 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
     1381 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
     1441 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
     1501 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
     1561 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
     1621 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
     1681 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
     1741 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
     1801 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
     1861 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
     1921 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1981 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2041 cactggctca ccttcgggtg ggcctttctg cgtttata
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