LOCUS       BBa_K2120318             862 bp    DNA     linear       25-May-2024
DEFINITION  J23109+B0032+tetR+B0015
ACCESSION   BBa_K2120318
VERSION     BBa_K2120318.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     RBS             36..48
                     /label=RBS-3\Weak
     RBS             42..45
                     /label=RBS
     misc_feature    36..48
                     /label=BBa_B0032
     RBS             36..48
                     /label=BBa_B0032
     CDS             52..668
                     /label=tetR
     misc_feature    669..702
                     /label=SsrA
     misc_feature    709..733
                     /label=Help:Barcodes
     CDS             49..733
                     /label=BBa_C0040
     stem_loop       745..788
                     /label=stem_loop
     misc_feature    734..813
                     /label=BBa_B0010
     terminator      734..813
                     /label=BBa_B0010
     stem_loop       829..848
                     /label=T7 TE
     misc_feature    855..855
                     /label=stop
     polyA_site      849..862
                     /label=polya
     misc_feature    822..862
                     /label=BBa_B0012
     terminator      822..862
                     /label=BBa_B0012
     terminator      734..862
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctcaca caggaaagat gtccagatta
       61 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
      121 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat
      181 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat
      241 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa
      301 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg
      361 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt
      421 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc
      481 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat
      541 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca
      601 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa
      661 agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc
      721 tagtgtagat cacccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      781 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      841 ggtgggcctt tctgcgttta ta
//