LOCUS       BBa_K2120401            1447 bp    DNA     linear       13-May-2024
DEFINITION  B0015+tetR+B0034+J23119+pTet+B0032+mazF+B0015
ACCESSION   BBa_K2120401
VERSION     BBa_K2120401.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    831..842
                     /label=inverse RBS
     RBS             831..842
                     /label=BBa_K1045010
     promoter        851..885
                     /label=promoter
     promoter        851..885
                     /label=BBa_K2120101
     misc_feature    894..912
                     /label=TetR 1
     misc_feature    894..947
                     /label=BBa_R0040
     promoter        913..918
                     /label=-35
     misc_feature    919..937
                     /label=TetR 2
     promoter        936..941
                     /label=-10
     promoter        894..947
                     /label=BBa_R0040
     RBS             956..968
                     /label=RBS-3\Weak
     RBS             962..965
                     /label=RBS
     misc_feature    956..968
                     /label=BBa_B0032
     RBS             956..968
                     /label=BBa_B0032
     CDS             975..1310
                     /label=BBa_K2120008
     stem_loop       1330..1373
                     /label=stem_loop
     misc_feature    1319..1398
                     /label=BBa_B0010
     terminator      1319..1398
                     /label=BBa_B0010
     stem_loop       1414..1433
                     /label=T7 TE
     misc_feature    1440..1440
                     /label=stop
     polyA_site      1434..1447
                     /label=polya
     misc_feature    1407..1447
                     /label=BBa_B0012
     terminator      1407..1447
                     /label=BBa_B0012
     terminator      1319..1447
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggtg atctacacta gcactatcag tgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag cggacccact ttcacattta agttgttttt ctaatccgca
      241 tatgatcaat tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc
      301 gatagcttgt cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt
      361 agcgacttga tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct
      421 gagtgcatat aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt
      481 ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc
      541 gcgatgactt agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca
      601 gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa
      661 ggcgtcgagc aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc
      721 tagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc
      781 taatgcgctg ttaatcactt tacttttatc taatctggac attactagag tttctcctct
      841 tttactagag gctagcatta tacctaggac tgagctagct gtcaatacta gagtccctat
      901 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagtcaca
      961 caggaaagta ctagatggta agccgatacg tacccgatat gggcgatctg atttgggttg
     1021 attttgaccc gacaaaaggt agcgagcaag ctggacatcg tccagctgtt gtcctgagtc
     1081 ctttcatgta caacaacaaa acaggtatgt gtctgtgtgt tccttgtaca acgcaatcaa
     1141 aaggatatcc gttcgaagtt gttttatccg gtcaggaacg tgatggcgta gcgttagctg
     1201 atcaggtaaa aagtatcgcc tggcgggcaa gaggagcaac gaagaaagga acagttgccc
     1261 cagaggaatt acaactcatt aaagccaaaa ttaacgtact gattgggtag tactagagcc
     1321 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1381 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1441 gtttata
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