LOCUS       BBa_K2120402            1446 bp    DNA     linear       12-May-2024
DEFINITION  B0015+tetR+B0034+J23116+ptet+B0034+mazF+B0015
ACCESSION   BBa_K2120402
VERSION     BBa_K2120402.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    831..842
                     /label=inverse RBS
     RBS             831..842
                     /label=BBa_K1045010
     promoter        851..885
                     /label=BBa_K2120102
     misc_feature    894..912
                     /label=TetR 1
     misc_feature    894..947
                     /label=BBa_R0040
     promoter        913..918
                     /label=-35
     misc_feature    919..937
                     /label=TetR 2
     promoter        936..941
                     /label=-10
     promoter        894..947
                     /label=BBa_R0040
     misc_feature    960..963
                     /label=conserved
     RBS             956..967
                     /label=BBa_B0034
     CDS             974..1309
                     /label=BBa_K2120008
     stem_loop       1329..1372
                     /label=stem_loop
     misc_feature    1318..1397
                     /label=BBa_B0010
     terminator      1318..1397
                     /label=BBa_B0010
     stem_loop       1413..1432
                     /label=T7 TE
     misc_feature    1439..1439
                     /label=stop
     polyA_site      1433..1446
                     /label=polya
     misc_feature    1406..1446
                     /label=BBa_B0012
     terminator      1406..1446
                     /label=BBa_B0012
     terminator      1318..1446
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggtg atctacacta gcactatcag tgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag cggacccact ttcacattta agttgttttt ctaatccgca
      241 tatgatcaat tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc
      301 gatagcttgt cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt
      361 agcgacttga tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct
      421 gagtgcatat aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt
      481 ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc
      541 gcgatgactt agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca
      601 gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa
      661 ggcgtcgagc aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc
      721 tagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc
      781 taatgcgctg ttaatcactt tacttttatc taatctggac attactagag tttctcctct
      841 tttactagag gctagcatag tccctaggac tgagctagct gtcaatacta gagtccctat
      901 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga
      961 ggagaaatac tagatggtaa gccgatacgt acccgatatg ggcgatctga tttgggttga
     1021 ttttgacccg acaaaaggta gcgagcaagc tggacatcgt ccagctgttg tcctgagtcc
     1081 tttcatgtac aacaacaaaa caggtatgtg tctgtgtgtt ccttgtacaa cgcaatcaaa
     1141 aggatatccg ttcgaagttg ttttatccgg tcaggaacgt gatggcgtag cgttagctga
     1201 tcaggtaaaa agtatcgcct ggcgggcaag aggagcaacg aagaaaggaa cagttgcccc
     1261 agaggaatta caactcatta aagccaaaat taacgtactg attgggtagt actagagcca
     1321 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1381 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1441 tttata
//