LOCUS       BBa_K2120405            1531 bp    DNA     linear       13-May-2024
DEFINITION  B0015+cI+B0034+J23119+pR+B0034+mazF+B0015
ACCESSION   BBa_K2120405
VERSION     BBa_K2120405.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     CDS             138..912
                     /label=BBa_K2120105
     misc_feature    921..932
                     /label=inverse RBS
     RBS             921..932
                     /label=BBa_K1045010
     promoter        941..975
                     /label=promoter
     promoter        941..975
                     /label=BBa_K2120101
     promoter        1021..1026
                     /label=-10
     promoter        998..1003
                     /label=-35
     misc_feature    1008..1024
                     /label=OR1
     misc_feature    984..1000
                     /label=OR2
     misc_feature    984..1032
                     /label=BBa_R0051
     promoter        984..1032
                     /label=BBa_R0051
     misc_feature    1045..1048
                     /label=conserved
     RBS             1041..1052
                     /label=BBa_B0034
     CDS             1059..1394
                     /label=BBa_K2120008
     stem_loop       1414..1457
                     /label=stem_loop
     misc_feature    1403..1482
                     /label=BBa_B0010
     terminator      1403..1482
                     /label=BBa_B0010
     stem_loop       1498..1517
                     /label=T7 TE
     misc_feature    1524..1524
                     /label=stop
     polyA_site      1518..1531
                     /label=polya
     misc_feature    1491..1531
                     /label=BBa_B0012
     terminator      1491..1531
                     /label=BBa_B0012
     terminator      1403..1531
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcg atctacacta gcactatcag cgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag cgccaaacgt ctcttcaggc cactgactag cgataacttt
      241 ccccacaacg gaacaactct cattgcatgg gatcattggg tactgtgggt ttagtggttg
      301 taaaaacacc tgaccgctat ccctgatcag tttcttgaag gtaaactcat cacccccaag
      361 tctggctatg cagaaatcac ctggctcaac agcctgctca gggtcaacga gaattaacat
      421 tccgtcagga aagcttggct tggagcctgt tggtgcggtc atggaattac cttcaacctc
      481 aagccagaat gcagaatcac tggctttttt ggttgtgctt acccatctct ccgcatcacc
      541 tttggtaaag gttctaagct caggtgagaa catccctgcc tgaacatgag aaaaaacagg
      601 gtactcatac tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat
      661 ttctctggcg attgaagggc taaattcttc aacgctaact ttgagaattt ttgcaagcaa
      721 tgcggcgtta taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg
      781 ccccatcccc atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt
      841 ttcataaatt gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt
      901 ttttgtgctc attactagag tttctcctct tttactagag gctagcatta tacctaggac
      961 tgagctagct gtcaatacta gagtaacacc gtgcgtgttg actattttac ctctggcggt
     1021 gataatggtt gctactagag aaagaggaga aatactagat ggtaagccga tacgtacccg
     1081 atatgggcga tctgatttgg gttgattttg acccgacaaa aggtagcgag caagctggac
     1141 atcgtccagc tgttgtcctg agtcctttca tgtacaacaa caaaacaggt atgtgtctgt
     1201 gtgttccttg tacaacgcaa tcaaaaggat atccgttcga agttgtttta tccggtcagg
     1261 aacgtgatgg cgtagcgtta gctgatcagg taaaaagtat cgcctggcgg gcaagaggag
     1321 caacgaagaa aggaacagtt gccccagagg aattacaact cattaaagcc aaaattaacg
     1381 tactgattgg gtagtactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1441 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1501 tcaccttcgg gtgggccttt ctgcgtttat a
//