LOCUS       BBa_K2130003            1406 bp    DNA     linear       29-May-2024
DEFINITION  ZsGreen(GFP) reporter with 2X dCas9-inducible promoter
ACCESSION   BBa_K2130003
VERSION     BBa_K2130003.1  
FEATURES             Location/Qualifiers
     promoter        13..38
                     /label=dCas9 binding site
     CDS             444..1139
                     /label=ZsGreen(GFP)
     polyA_site      1167..1394
                     /label=bGH pA
     promoter        79..104
                     /label=dCas9 binding site
     promoter        107..422
                     /label=TRE
ORIGIN
        1 attacagggc ccgtcccctc caccccacag tggggagtag agagacagta catgccctgg
       61 gagttccagt ttatcgatgt cccctccacc ccacagtggg gagtctcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgatgtcga
      181 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat
      301 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
      361 gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg
      421 cctggagaag gatccgcgac cgcatggccc agtccaagca cggcctgacc aaggagatga
      481 ccatgaagta ccgcatggag ggctgcgtgg acggccacaa gttcgtgatc accggcgagg
      541 gcatcggcta ccccttcaag ggcaagcagg ccatcaacct gtgcgtggtg gagggcggcc
      601 ccttgccctt cgccgaggac atcttgtccg ccgccttcat gtacggcaac cgcgtgttca
      661 ccgagtaccc ccaggacatc gtcgactact tcaagaactc ctgccccgcc ggctacacct
      721 gggaccgctc cttcctgttc gaggacggcg ccgtgtgcat ctgcaacgcc gacatcaccg
      781 tcagcgtgga ggagaactgc atgtaccacg agtccaagtt ctacggcgtg aacttccccg
      841 ccgacggccc cgtgatgaag aagatgaccg acaactggga gccctcctgc gagaagatca
      901 tccccgtgcc caagcagggc atcttgaagg gcgacgtcag catgtacctg ctgctgaagg
      961 acggtggccg cttgcgctgc cagttcgaca ccgtgtacaa ggccaagtcc gtgccccgca
     1021 agatgcccga ctggcacttc atccagcaca agctgacccg cgaggaccgc agcgacgcca
     1081 agaaccagaa gtggcacctg accgagcacg ccatcgcctc cggctccgcc ttgccctgaa
     1141 cgcgtgtctg gaccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     1201 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1261 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     1321 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     1381 ggtgggctct atggcttctg aggcgg
//