LOCUS       BBa_K2130005            3324 bp    DNA     linear       29-May-2024
DEFINITION  NmCas9
ACCESSION   BBa_K2130005
VERSION     BBa_K2130005.1  
FEATURES             Location/Qualifiers
     CDS             3277..3324
                     /label=NLS
     CDS             7..27
                     /label=NLS
     misc_feature    28..3270
                     /label=Nm-Cas9
ORIGIN
        1 atggtgccta agaagaagag aaaggtggct gccttcaaac ctaattcaat caactacatc
       61 ctcggcctcg atatcggcat cgcatccgtc ggctgggcga tggtagaaat tgacgaagaa
      121 gaaaacccca tccgcctgat tgatttgggc gtgcgcgtat ttgagcgtgc cgaagtaccg
      181 aaaacaggcg actcccttgc catggcaagg cgtttggcgc gcagtgttcg ccgcctgacc
      241 cgccgtcgcg cccaccgcct gcttcggacc cgccgcctat tgaaacgcga aggcgtatta
      301 caagccgcca attttgacga aaacggcttg attaaatcct taccgaatac accatggcaa
      361 cttcgcgcag ccgcattaga ccgcaaactg acgcctttag agtggtcggc agtcttgttg
      421 catttaatca aacatcgcgg ctatttatcg caacggaaaa acgagggcga aactgccgat
      481 aaggagcttg gcgctttgct taaaggcgta gccggcaatg cccatgcctt acagacaggc
      541 gatttccgca caccggccga attggcttta aataaatttg agaaagaaag cggccatatc
      601 cgcaatcagc gcagcgatta ttcgcatacg ttcagccgca aagatttaca ggcggagctg
      661 attttgctgt ttgaaaaaca aaaagaattt ggcaatccgc atgtttcagg cggccttaaa
      721 gaaggtattg aaaccctact gatgacgcaa cgccctgccc tgtccggcga tgccgttcaa
      781 aaaatgttgg ggcattgcac cttcgaaccg gcagagccga aagccgctaa aaacacctac
      841 acagccgaac gtttcatctg gctgaccaag ctgaacaacc tgcgtatttt agagcaaggc
      901 agcgagcggc cattgaccga taccgaacgc gccacgctta tggacgagcc atacagaaaa
      961 tccaaactga cttacgcaca agcccgtaag ctgctgggtt tagaagatac cgcctttttc
     1021 aaaggcttgc gctatggtaa agacaatgcc gaagcctcaa cattgatgga aatgaaggcc
     1081 taccatgcca tcagccgtgc actggaaaaa gaaggattga aagacaaaaa atccccatta
     1141 aacctttctc ccgaattaca agacgaaatc ggcacggcat tctccctgtt caaaaccgat
     1201 gaagacatta caggccgtct gaaagaccgt atacagcccg aaatcttaga agcgctgttg
     1261 aaacacatca gcttcgataa gttcgtccaa atttccttga aagcattgcg ccgaattgtg
     1321 cctctaatgg aacaaggcaa acgttacgat gaagcctgcg ccgaaatcta cggagaccat
     1381 tacggcaaga agaatacgga agaaaagatt tatctgccgc cgattcccgc cgacgaaatc
     1441 cgcaaccccg tcgtcttgcg cgccttatct caagcacgta aggtcattaa cggcgtggta
     1501 cgccgttacg gctccccagc tcgtatccat attgaaactg caagggaagt aggtaaatcg
     1561 tttaaagacc gcaaagaaat tgagaaacgc caagaagaaa accgcaaaga ccgggaaaaa
     1621 gccgccgcca aattccgaga gtatttcccc aattttgtcg gagaacccaa atccaaagat
     1681 attctgaaac tgcgcctgta cgagcaacaa cacggcaaat gcctgtattc gggcaaagaa
     1741 atcaacttag gccgtctgaa cgaaaaaggc tatgtcgaaa tcgaccatgc cctgccgttc
     1801 tcgcgcacat gggacgacag tttcaacaat aaagtactgg tattgggcag cgaaaaccaa
     1861 aacaaaggca atcaaacccc ttacgaatac ttcaacggca aagacaacag ccgcgaatgg
     1921 caggaattta aagcgcgtgt cgaaaccagc cgtttcccgc gcagtaaaaa acaacggatt
     1981 ctgctgcaaa aattcgatga agacggcttt aaagaacgca atctgaacga cacgcgctac
     2041 gtcaaccgtt tcctgtgtca atttgttgcc gaccgtatgc ggctgacagg taaaggcaag
     2101 aaacgtgtct ttgcatccaa cggacaaatt accaatctgt tgcgcggctt ttggggattg
     2161 cgcaaagtgc gtgcggaaaa cgaccgccat cacgccttgg acgccgtcgt cgttgcctgc
     2221 tcgaccgttg ccatgcagca gaaaattacc cgttttgtac gctataaaga gatgaacgcg
     2281 tttgacggta aaaccataga caaagaaaca ggagaagtgc tgcatcaaaa aacacacttc
     2341 ccacaacctt gggaattttt cgcacaagaa gtcatgattc gcgtcttcgg caaaccggac
     2401 ggcaaacccg aatttgaaga agccgatacc ctagaaaaac tgcgcacgtt gcttgccgaa
     2461 aaattatcat ctcgccccga agccgtacac gaatacgtta cgccactgtt tgtttcacgc
     2521 gcgcccaatc ggaagatgag cgggcaaggg catatggaga ccgtcaaatc cgccaaacga
     2581 ctggacgaag gcgtcagcgt gttgcgcgta ccgctgacac agttaaaact gaaagacttg
     2641 gaaaaaatgg tcaatcggga gcgcgaacct aagctatacg aagcactgaa agcacggctg
     2701 gaagcacata aagacgatcc tgccaaagcc tttgccgagc cgttttacaa atacgataaa
     2761 gcaggcaacc gcacccaaca ggtaaaagcc gtacgcgtag agcaagtaca gaaaaccggc
     2821 gtatgggtgc gcaaccataa cggtattgcc gacaacgcaa ccatggtgcg cgtagatgtg
     2881 tttgagaaag gcgacaagta ttatctggta ccgatttaca gttggcaggt agcgaaaggg
     2941 attttgccgg atagggctgt tgtacaagga aaagatgaag aagattggca acttattgat
     3001 gatagtttca actttaaatt ctcattacac cctaatgatt tagtcgaggt tataacaaaa
     3061 aaagctagaa tgtttggtta ctttgccagc tgccatcgag gcacaggtaa tatcaatata
     3121 cgcattcatg atcttgatca taaaattggc aaaaatggaa tactggaagg tatcggcgtc
     3181 aaaaccgccc tttcattcca aaaataccaa attgacgaac tgggcaaaga aatcagacca
     3241 tgccgtctga aaaaacgccc gcctgtccgt accggtaaaa ggccggcggc cacgaaaaag
     3301 gccggccagg caaaaaagaa aaag
//