LOCUS       BBa_K2145002            2014 bp    DNA     linear       01-Jun-2024
DEFINITION  GFP/RFP reporter with spacer (500bp)
ACCESSION   BBa_K2145002
VERSION     BBa_K2145002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI
     misc_feature    173..173
                     /label=start
     misc_feature    209..220
                     /label=BBa_B0034
     misc_feature    213..216
                     /label=conserved
     misc_feature    227..907
                     /label=BBa_E1010
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=barcode
     misc_feature    941..1020
                     /label=BBa_B0010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    1029..1069
                     /label=BBa_B0012
     stem_loop       1036..1055
                     /label=T7 TE
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1062..1062
                     /label=stop
     misc_feature    1..1069
                     /label=BBa_J04450
     misc_feature    1078..1096
                     /label=TetR 1
     misc_feature    1078..1131
                     /label=BBa_R0040
     promoter        1097..1102
                     /label=-35
     misc_feature    1103..1121
                     /label=TetR 2
     promoter        1120..1125
                     /label=-10
     misc_feature    1140..1151
                     /label=BBa_B0034
     misc_feature    1144..1147
                     /label=conserved
     misc_feature    1158..1877
                     /label=BBa_E0040
     misc_feature    1886..1965
                     /label=BBa_B0010
     stem_loop       1897..1940
                     /label=stem_loop
     misc_feature    1974..2014
                     /label=BBa_B0012
     stem_loop       1981..2000
                     /label=T7 TE
     polyA_site      2001..2014
                     /label=polya
     misc_feature    2007..2007
                     /label=stop
     misc_feature    1078..2014
                     /label=BBa_I13522
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc
     1081 ctatcagtga tagagattga catccctatc agtgatagag atactgagca ctactagaga
     1141 aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
     1201 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     1261 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1321 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
     1381 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     1441 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
     1501 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     1561 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     1621 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     1681 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1741 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1801 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1861 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1921 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1981 ggctcacctt cgggtgggcc tttctgcgtt tata
//