BBa_B0033 1 BBa_B0033 RBS.4 (weaker) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-3&quot; in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7028 1 BBa_B0033 range7028 1 1 11 annotation1713 1 RBS-4\Weaker range1713 1 1 11 annotation1714 1 RBS range1714 1 7 10 BBa_K2150010 1 BBa_K2150010 This part consists of a gene encoding toxin 134 with a LacI gene, a Ptac promoter included 2016-10-05T11:00:00Z 2016-10-13T10:02:17Z This gene is acquired by PCR from genome of Bacillus subtilis This part contain sequence encoding EndoA, a toxin gene in bacteria. true false _2620_ 29734 29734 9 false We used RBS BBa_B0033 to reduce leakage, and there is a RiboJ (an insulator element) directly upstream to the CDS in order to reduce the background noise. false Zepeng Mu component2486562 1 BBa_B0033 annotation2486562 1 BBa_B0033 range2486562 1 1 11 BBa_K2150010_sequence 1 tcacacaggac BBa_B0033_sequence 1 tcacacaggac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z