LOCUS       BBa_K215002              946 bp    DNA     linear       11-May-2024
DEFINITION  pLac+RBS+Secretion Signal and Streptavidin Binding Tags
ACCESSION   BBa_K215002
VERSION     BBa_K215002.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..129
                     /label=nanotag
     misc_feature    130..138
                     /label=GSA linker
     misc_feature    139..156
                     /label=His tag
     misc_feature    157..165
                     /label=GSA linker
     misc_feature    166..185
                     /label=TEV protease site
     misc_feature    186..195
                     /label=GSA linker
     misc_feature    196..201
                     /label=NheI
     misc_feature    202..210
                     /label=GSA linker
     misc_feature    211..231
                     /label=TEV protease site
     misc_feature    232..240
                     /label=GSA linker
     misc_feature    241..258
                     /label=His tag
     misc_feature    259..267
                     /label=GSA linker
     misc_feature    268..816
                     /label=Secretion tag
     misc_feature    817..946
                     /label=terminator (B0015)
     misc_feature    82..946
                     /label=BBa_K215001
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggacgtt gaagcatggc tgggtgcccg tgtaccgctg
      121 gttgaaacgg gcagcgcaca tcaccaccat catcatggtt ccgccgaaaa cctctacttt
      181 cagggcggct ctgctgctag cggttctgcc gagaatctgt acttccaagg tggctccgct
      241 catcaccacc atcatcacgg tagcgcaggc ggcactgaca cctttgactt ctctggttac
      301 tctaacaatc agcgtatcaa cctgaacgag ggttctttct ctgacgttgg tggtctgaag
      361 ggcaacgttt ctatcgcgca tggtgttacc atcgaaaacg cgatcggtgg ttctggtaac
      421 gacatcctga tcggtaatgg cgcggataac atcctccagg gtggtgcggg tgacgacgtt
      481 ctgtacggtt ctacgggtgc ggacacgctg acgggtggcg ctggtcgtga cattttcgta
      541 tatggtagcg gtcaagactc taccgtttct gcatacgact ggatcacgga ttttcagacg
      601 ggtatcgaca aaatcgacct gtccgctttc cgtaatgaag gtcagctgtc ttttgttcag
      661 gaccagttta ctggtaaagg ccaggaggtt atgctgcaat gggacgcggc gaactctacg
      721 accaatctgt ggctgcacga agcgggtcac tcttctgttg actttctcgt tcgtatcgtt
      781 ggccagaccg cgcagagcga cattatcgtc taataaccag gcatcaaata aaacgaaagg
      841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatat
//