BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K215001 1 BBa_K215001 Secretion Signal and Streptavidin Binding Tags 2009-09-29T11:00:00Z 2015-05-08T01:11:29Z Nanotag (bases 1-48): From Lamla and Erdmann (http://www.ncbi.nlm.nih.gov/pubmed/14680960). Secretion tag (last 181 AA): Gene Bank ID Erwinia chrysanthemi M60395.1 This part is the vector into which afp is inserted to tag it for purification using the IPP system. It contains an NheI restriction site that allows for the insertion of the desired protein coding sequence (NheI is compatible with XbaI and SpeI). This Target Vector part contains two tags: a secretion tag that causes the protein to be exported to the extracellular space, and a nanotag that causes the protein to bind to stretavidin (or some analog). For this part to function properly, it must be combined with a promoter and RBS, and used in E.coli strain UW5alpha, which has two other plasmids containing the other genes required for proper secretion and display of the protein to be purified on the outer membrane. false false _320_ 0 2811 9 It's complicated false NheI was chosen as the protein insertion site because it is compatible with digested XbaI and SpeI restriction sites. The TEV sites and HIS tags flanking the NheI site are present to separate the inserted protein from the added tags. GSA linkers were used to separate the various features of the part. false Jeff Nivala annotation2027598 1 GSA linker range2027598 1 178 186 annotation2027587 1 nanotag range2027587 1 1 48 annotation2027600 1 terminator (B0015) range2027600 1 736 865 annotation2027593 1 NheI range2027593 1 115 120 annotation2027595 1 TEV protease site range2027595 1 130 150 annotation2027594 1 GSA linker range2027594 1 121 129 annotation2027596 1 GSA linker range2027596 1 151 159 annotation2027590 1 GSA linker range2027590 1 76 84 annotation2027597 1 His tag range2027597 1 160 177 annotation2027592 1 GSA linker range2027592 1 105 114 annotation2027591 1 TEV protease site range2027591 1 85 104 annotation2027599 1 Secretion tag range2027599 1 187 735 annotation2027589 1 His tag range2027589 1 58 75 annotation2027588 1 GSA linker range2027588 1 49 57 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation1999 1 lac O1 range1999 1 3 19 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2000 1 -35 range2000 1 20 25 annotation2001 1 lac O1 range2001 1 26 42 annotation2002 1 -10 range2002 1 43 48 BBa_K215002 1 BBa_K215002 pLac+RBS+Secretion Signal and Streptavidin Binding Tags 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z nnn nnn false false _320_ 0 2811 9 It's complicated true nnn false Jeff Nivala component2027627 1 BBa_B0034 component2027621 1 BBa_R0011 component2027642 1 BBa_K215001 annotation2027621 1 BBa_R0011 range2027621 1 1 54 annotation2027627 1 BBa_B0034 range2027627 1 64 75 annotation2027642 1 BBa_K215001 range2027642 1 82 946 BBa_B0034_sequence 1 aaagaggagaaa BBa_K215002_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggacgttgaagcatggctgggtgcccgtgtaccgctggttgaaacgggcagcgcacatcaccaccatcatcatggttccgccgaaaacctctactttcagggcggctctgctgctagcggttctgccgagaatctgtacttccaaggtggctccgctcatcaccaccatcatcacggtagcgcaggcggcactgacacctttgacttctctggttactctaacaatcagcgtatcaacctgaacgagggttctttctctgacgttggtggtctgaagggcaacgtttctatcgcgcatggtgttaccatcgaaaacgcgatcggtggttctggtaacgacatcctgatcggtaatggcgcggataacatcctccagggtggtgcgggtgacgacgttctgtacggttctacgggtgcggacacgctgacgggtggcgctggtcgtgacattttcgtatatggtagcggtcaagactctaccgtttctgcatacgactggatcacggattttcagacgggtatcgacaaaatcgacctgtccgctttccgtaatgaaggtcagctgtcttttgttcaggaccagtttactggtaaaggccaggaggttatgctgcaatgggacgcggcgaactctacgaccaatctgtggctgcacgaagcgggtcactcttctgttgactttctcgttcgtatcgttggccagaccgcgcagagcgacattatcgtctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat BBa_K215001_sequence 1 atggacgttgaagcatggctgggtgcccgtgtaccgctggttgaaacgggcagcgcacatcaccaccatcatcatggttccgccgaaaacctctactttcagggcggctctgctgctagcggttctgccgagaatctgtacttccaaggtggctccgctcatcaccaccatcatcacggtagcgcaggcggcactgacacctttgacttctctggttactctaacaatcagcgtatcaacctgaacgagggttctttctctgacgttggtggtctgaagggcaacgtttctatcgcgcatggtgttaccatcgaaaacgcgatcggtggttctggtaacgacatcctgatcggtaatggcgcggataacatcctccagggtggtgcgggtgacgacgttctgtacggttctacgggtgcggacacgctgacgggtggcgctggtcgtgacattttcgtatatggtagcggtcaagactctaccgtttctgcatacgactggatcacggattttcagacgggtatcgacaaaatcgacctgtccgctttccgtaatgaaggtcagctgtcttttgttcaggaccagtttactggtaaaggccaggaggttatgctgcaatgggacgcggcgaactctacgaccaatctgtggctgcacgaagcgggtcactcttctgttgactttctcgttcgtatcgttggccagaccgcgcagagcgacattatcgtctaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z