BBa_B0033 1 BBa_B0033 RBS.4 (weaker) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-3&quot; in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1713 1 RBS-4\Weaker range1713 1 1 11 annotation1714 1 RBS range1714 1 7 10 annotation7028 1 BBa_B0033 range7028 1 1 11 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_K1679038 1 BBa_K1679038 RiboJ 2015-09-17T11:00:00Z 2015-09-18T01:15:04Z Synthesized by Genewiz. After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme and an additional 23-nt hairpin immediately downstream to help expose the RBS. STRSV can cleaves the mRNA autocatalytically at a defined residue, which had the effect of cleaving extraneous RNA leaders that arose from transcription from promoters with different start sites.[1] Attributed to the biochemical function mentioned above, RiboJ has insulating capability. We use it to buffer synthetic circuits from genetic context. [1] Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142. false false _2097_ 25072 25072 9 false After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme and an additional 23-nt hairpin. Users can insert it into promoter and RBS to reduce noise caused by upstream sequence and stabilize mRNA. false Xihan Zhang BBa_K2150112 1 BBa_K2150112 1204 toxin tet 2016-10-12T11:00:00Z 2016-10-18T07:00:20Z a a false false _2620_ 29734 29734 9 false a false Zepeng Mu component2519923 1 BBa_B0033 component2519916 1 BBa_R0040 component2519927 1 BBa_K2150103 component2519921 1 BBa_K1679038 annotation2519916 1 BBa_R0040 range2519916 1 1 54 annotation2519923 1 BBa_B0033 range2519923 1 146 156 annotation2519921 1 BBa_K1679038 range2519921 1 63 137 annotation2519927 1 BBa_K2150103 range2519927 1 163 669 BBa_K2150103 1 N-acetyltr 1204 toxin CDS 2016-10-11T11:00:00Z 2016-10-18T06:28:40Z a a false false _2620_ 29734 29734 9 false a false Zepeng Mu annotation2519780 1 double stop codon range2519780 1 502 506 annotation2519779 1 CDS of N-acetyltransferase GCN5 range2519779 1 1 501 BBa_B0033_sequence 1 tcacacaggac BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K2150112_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagttaaacaaaattatttgtagaggctgtttcgtcctcacggactcatcagaccggaaagcacatccggtgacagcttactagagtcacacaggactactagatgagttccacgatgtttgatgttatgcaactcaacgaagaacacgatcgtgattcgttctacagtggttcagaatttctagatggttactttcagagacaggttggtcaggatgttaggtgccgcgtagcagcttgttttgttgcgcttcatgatcaccgtgtagcgggatattatactctggctgctgccggtatccagttggccaacctcccgacagctactattaagaaattgccacgatacccaacagtgccggcagtacggatggggaggttggccgttgatcaggcatttagaaatcagggactcggtggagcattgctagctgatgctattgagaggaccattcgtgccgaaattgcatcttatgcgctgcttgttgacgcaaaagatgataatgcagtgcaattttaccgacatcatggttttattgctttacccgattcgccattcacattattccttcctctcatcgctgtagataaaaaaggtaaaaaataataa BBa_K2150103_sequence 1 atgagttccacgatgtttgatgttatgcaactcaacgaagaacacgatcgtgattcgttctacagtggttcagaatttctagatggttactttcagagacaggttggtcaggatgttaggtgccgcgtagcagcttgttttgttgcgcttcatgatcaccgtgtagcgggatattatactctggctgctgccggtatccagttggccaacctcccgacagctactattaagaaattgccacgatacccaacagtgccggcagtacggatggggaggttggccgttgatcaggcatttagaaatcagggactcggtggagcattgctagctgatgctattgagaggaccattcgtgccgaaattgcatcttatgcgctgcttgttgacgcaaaagatgataatgcagtgcaattttaccgacatcatggttttattgctttacccgattcgccattcacattattccttcctctcatcgctgtagataaaaaaggtaaaaaataataa BBa_K1679038_sequence 1 ttaaacaaaattatttgtagaggctgtttcgtcctcacggactcatcagaccggaaagcacatccggtgacagct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z