LOCUS       BBa_K2150115             382 bp    DNA     linear       14-Jun-2024
DEFINITION  6249 antitoxin tet
ACCESSION   BBa_K2150115
VERSION     BBa_K2150115.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     promoter        63..137
                     /label=BBa_K1679038
     RBS             146..156
                     /label=RBS-4\Weaker
     RBS             152..155
                     /label=RBS
     misc_feature    146..156
                     /label=BBa_B0033
     RBS             146..156
                     /label=BBa_B0033
     CDS             165..376
                     /label=antitoxin ParD2 CDS
     misc_feature    377..382
                     /label=double stop codon
     CDS             165..382
                     /label=BBa_K2150106
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agttaaacaa aattatttgt agaggctgtt tcgtcctcac ggactcatca gaccggaaag
      121 cacatccggt gacagcttac tagagtcaca caggactact agagtggtgg tcaaccgggc
      181 attgctggcg agcgtcgacg cactgtcgcg tgatgagcag attgagctcg tcgagcacat
      241 caacggaaac ctagccgagg gcatgcatat cagcgaggcc aaccaggcgc tcatcgaagc
      301 gcgggccaat gacaccgacg atgctcattg gtccaccatt gatgacttcg acaagcggat
      361 ccgcgcccgg ctcggataat aa
//