LOCUS       BBa_K2150117            1775 bp    DNA     linear       01-Jun-2024
DEFINITION  6249 antitoxin IPTG
ACCESSION   BBa_K2150117
VERSION     BBa_K2150117.1  
FEATURES             Location/Qualifiers
     CDS             1..1082
                     /label=lacIq
     promoter        1325..1352
                     /label=Ptac
     promoter        1..1447
                     /label=BBa_K180000
     promoter        1456..1530
                     /label=BBa_K1679038
     RBS             1539..1549
                     /label=RBS-4\Weaker
     RBS             1545..1548
                     /label=RBS
     misc_feature    1539..1549
                     /label=BBa_B0033
     RBS             1539..1549
                     /label=BBa_B0033
     CDS             1558..1769
                     /label=antitoxin ParD2 CDS
     misc_feature    1770..1775
                     /label=double stop codon
     CDS             1558..1775
                     /label=BBa_K2150106
ORIGIN
        1 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 tgagcgcaac gcaattaatg taagttagct cactcattag gcacaattct catgtttgac
     1141 agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct
     1201 gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc
     1261 cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg
     1321 agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt
     1381 tcacacagga aacagccagt ccgtttaggt gttttcacga gcacttcacc aacaaggacc
     1441 atagcattac tagagttaaa caaaattatt tgtagaggct gtttcgtcct cacggactca
     1501 tcagaccgga aagcacatcc ggtgacagct tactagagtc acacaggact actagagtgg
     1561 tggtcaaccg ggcattgctg gcgagcgtcg acgcactgtc gcgtgatgag cagattgagc
     1621 tcgtcgagca catcaacgga aacctagccg agggcatgca tatcagcgag gccaaccagg
     1681 cgctcatcga agcgcgggcc aatgacaccg acgatgctca ttggtccacc attgatgact
     1741 tcgacaagcg gatccgcgcc cggctcggat aataa
//