LOCUS       BBa_K2150120             906 bp    DNA     linear       01-Jun-2024
DEFINITION  136 toxin aTc
ACCESSION   BBa_K2150120
VERSION     BBa_K2150120.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     promoter        63..137
                     /label=BBa_K1679038
     RBS             146..156
                     /label=RBS-4\Weaker
     RBS             152..155
                     /label=RBS
     misc_feature    146..156
                     /label=BBa_B0033
     RBS             146..156
                     /label=BBa_B0033
     CDS             163..906
                     /label=BBa_K2150102
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agttaaacaa aattatttgt agaggctgtt tcgtcctcac ggactcatca gaccggaaag
      121 cacatccggt gacagcttac tagagtcaca caggactact agatggatga cattcgcaag
      181 attgaacgat tagcggcagc ttcatggccg gcttattttc aaaaaagcat agggaaatgg
      241 cttctcagag cgaattttgg cgtaaccaaa agggcaaata gcgtatggac gtctgcggat
      301 atgcctgaag gggattttca gctagaggcc gaactttttt atcaaagttt aggcttacct
      361 gtttgttttc atattagcaa cgcatcaccg aaagggcttg atgacgcatt ggcagacagc
      421 cgttatgaaa aggtagacga gtgcttccag atgacagcgt tgtgccggtc aattatgagc
      481 cgaacaaatg acaacagccg atttacatac aagtgggaac aagagccatc atctgtttgg
      541 atagatgaat ttattcaatt agaagggttt tcacccgagc gtcacaaagg ttacaaacac
      601 atattcgaac gcatgccgcc gtgtaaaact tttttcaaga tgtatgacaa agaaagcctg
      661 acagcgcttg gcacggtttc tgtcattgat ggttatggcg gacttagtaa tatcgttgta
      721 gctgaggagc acagaggaaa aggggcagga acccaagtga tcagggtgtt gactgaatgg
      781 gcaaaaaaca acggagcgga acgcatgttt ttacaagtga tgaaagaaaa tctggcggca
      841 gtttctttat atggaaaaat cggcttttcg cctatatcgg aacaccatta ccggattaaa
      901 agataa
//