LOCUS       BBa_K215200              297 bp    DNA     linear       13-May-2024
DEFINITION  Generic Surface Display Construct: IPTG Inducible Lpp + 1tmr OmpA 
            + NheI Site
ACCESSION   BBa_K215200
VERSION     BBa_K215200.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..168
                     /label=BBa_J36835 - Lpp Signaling Peptide
     misc_feature    175..237
                     /label=BBa_J36836 - 1tmr OmpA
     misc_feature    238..270
                     /label=GS Linker - GGGSGGGSGGG
     misc_feature    271..291
                     /label=TEV Site - ENLYFQG
     misc_feature    292..297
                     /label=NheI
     CDS             82..297
                     /label=BBa_K215250
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaagct actaaactgg tactgggcgc ggtaatcctg
      121 ggttctactc tgctggcagg ttgctccagc aacgctaaaa tcgatcagac tagaaacccg
      181 tatgttggct ttgaaatggg ttacgactgg ttaggtcgta tgccgtacaa aggcagcggt
      241 ggtggttctg gtggtggttc tggtggtggt gaaaacctgt attttcaggg tgctagc
//