LOCUS       BBa_K215201              576 bp    DNA     linear       12-May-2024
DEFINITION  Generic Surface Display Construct: IPTG Inducible Lpp + 5tmr OmpA 
            + NheI Site
ACCESSION   BBa_K215201
VERSION     BBa_K215201.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..168
                     /label=BBa_J36835 - Lpp Signaling Peptide
     misc_feature    175..516
                     /label=BBa_J36837 - 5tmr OmpA
     misc_feature    517..549
                     /label=GS Linker - GGGSGGGSGGG
     misc_feature    550..570
                     /label=TEV Site - ENLYFQG
     misc_feature    571..576
                     /label=NheI
     CDS             82..576
                     /label=BBa_K215251
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaagct actaaactgg tactgggcgc ggtaatcctg
      121 ggttctactc tgctggcagg ttgctccagc aacgctaaaa tcgatcagac tagaaacccg
      181 tatgttggct ttgaaatggg ttacgactgg ttaggtcgta tgccgtacaa aggcagcgtt
      241 gaaaacggtg catacaaagc tcagggcgtt caactgaccg ctaaactggg ttacccaatc
      301 actgacgacc tggacatcta cactcgtctg ggtggcatgg tatggcgtgc agacactaaa
      361 tccaacgttt atggtaaaaa ccacgacacc ggcgtttctc cggtcttcgc tggcggtgtt
      421 gagtacgcga tcactcctga aatcgctacc cgtctggaat accagtggac caacaacatc
      481 ggtgacgcac acaccatcgg cactcgtccg gacaacggtg gtggttctgg tggtggttct
      541 ggtggtggtg aaaacctgta ttttcagggt gctagc
//