| Types | DnaRegion
|
| Roles | Translational_Unit
engineered_region
|
| Sequences | BBa_K216006_sequence (Version 1)
|
Description
This is the coding sequence for the enzyme pentaerythritol tetranitrate reductase (PETN reductase) of Enterobacter cloacae PB2. This enzyme reductively denitrates nitrate esters such as PETN (pentaerythritol tetranitrate, penthrite) GTN (glycerol trinitrate, nitroglycerine) and EGDN (ethylene glycol dinitrate) yielding the alcohol and nitrite ion. It can also reductively attack nitroaromatic compounds such as TNT (2,4,6-trinitrotoluene) forming initially hydride-Meisenheimer complexes which break down via an unknown route to yield nitrite and non-aromatic products. Reference: Binks, P.R., French, C.E., Nicklin, S., & Bruce, N.C. (1996). Degradation of pentaerythritol tetranitrate by Enterobacter cloacae PB2. Applied and Environmental Microbiology 62(4), 1214-1219. Reference 2: French,C.E., Nicklin,S. and Bruce,N.C. 1996. Sequence and properties of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2. J. Bacteriol. 178 (22), 6623-6627 (1996).
Notes
No special considerations, but due to an error, the primers were actually designed based on the homologous gene of another strain of Enterobacter cloacae, which has a very similar coding sequence but different sequence in the upstream region. The sequence shown here is the correct sequence determined by sequencing the construct.
Source
This part was amplified from plasmid pONR1 which bears the PETN reductase gene originally cloned from Enterobacter cloacae PB2.
