BBa_K216012 1 BBa_K216012 PompC+EYFP+PETN reductase 2009-10-15T11:00:00Z 2015-05-08T01:11:30Z Made by joining BBa_K216011 to BBa_K216006. This is the ompC promoter, controlled by response regulator OmpR and sensor kinase EnvZ, joined to Enhanced Yellow Fluorescent Protein and PETN reductase, an enzyme which degrades nitrate ester and nitroaromatic explosives yielding nitrite. false false _317_ 0 837 163 It's complicated true No special considerations. false Edinburgh iGEM 2009 component2047052 1 BBa_E0030 component2047057 1 BBa_K216006 component2047051 1 BBa_B0034 component2047049 1 BBa_R0082 annotation2047051 1 BBa_B0034 range2047051 1 117 128 annotation2047057 1 BBa_K216006 range2047057 1 866 1974 annotation2047049 1 BBa_R0082 range2047049 1 1 108 annotation2047052 1 BBa_E0030 range2047052 1 135 857 BBa_K216006 1 BBa_K216006 pentaerythritol tetranitrate reductase with native rbs 2009-10-15T11:00:00Z 2015-05-08T01:11:30Z This part was amplified from plasmid pONR1 which bears the PETN reductase gene originally cloned from ''Enterobacter cloacae'' PB2. This is the coding sequence for the enzyme pentaerythritol tetranitrate reductase (PETN reductase) of ''Enterobacter cloacae'' PB2. This enzyme reductively denitrates nitrate esters such as PETN (pentaerythritol tetranitrate, penthrite) GTN (glycerol trinitrate, nitroglycerine) and EGDN (ethylene glycol dinitrate) yielding the alcohol and nitrite ion. It can also reductively attack nitroaromatic compounds such as TNT (2,4,6-trinitrotoluene) forming initially hydride-Meisenheimer complexes which break down via an unknown route to yield nitrite and non-aromatic products. Reference: Binks, P.R., French, C.E., Nicklin, S., & Bruce, N.C. (1996). Degradation of pentaerythritol tetranitrate by ''Enterobacter cloacae'' PB2. Applied and Environmental Microbiology 62(4), 1214-1219. Reference 2: French,C.E., Nicklin,S. and Bruce,N.C. 1996. Sequence and properties of pentaerythritol tetranitrate reductase from ''Enterobacter cloacae'' PB2. J. Bacteriol. 178 (22), 6623-6627 (1996). false false _317_ 0 837 163 It's complicated true No special considerations, but due to an error, the primers were actually designed based on the homologous gene of another strain of ''Enterobacter cloacae'', which has a very similar coding sequence but different sequence in the upstream region. The sequence shown here is the correct sequence determined by sequencing the construct. false Edinburgh iGEM 2009 annotation2046801 1 RBS range2046801 1 1 3 annotation2046802 1 Start range2046802 1 9 11 annotation2046804 1 PETN reductase coding region range2046804 1 9 1106 annotation2046803 1 stop range2046803 1 1104 1109 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E0030 1 eyfp enhanced yellow fluorescent protein derived from A. victoria GFP 2004-03-02T12:00:00Z 2015-08-31T04:07:25Z Modificaitons to Clontech EYFP by Reshma Shetty Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff BBa_R0082 1 OmpR Promoter (OmpR, positive) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z NC_000193 E. coli K12 Released HQ 2013 Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. false false _1_ 0 24 7 In stock false The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown. true Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) annotation301154 1 C1 OmpR range301154 1 13 30 annotation301166 1 -35 range301166 1 75 80 annotation301156 1 C3 OmpR range301156 1 54 71 annotation301167 1 -10 range301167 1 98 103 annotation301155 1 C2 OmpR range301155 1 34 51 BBa_K216012_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagaggagtcgttatgtcagctgaaaagctgtttaccccactgaaagtgggtgccgttactgccccaaaccgcgtgtttatggccccacttacccgtctgcgcagcatcgagccgggcgatatcccaacgccattgatgggtgagtattaccgccagcgcgccagcgcgggcctgattatctccgaagccacgcagatttctgctcaggcaaaaggctacgccggtgcaccgggtctgcacagcccggaacagatcgccgcgtggaaaaaaatcaccgcaggcgtgcatgctgaagatggccgtattgcggttcagctgtggcacaccggtcgtatctcacacagcagcatccagcctggcggtcaggcgccggtttctgcctctgccctgaacgccaatacccgcacttccctgcgcgatgaaaacggtaatgcgatccgcgtcgacaccaccacgccacgcgcgctggagctggacgagatcccgggtatcgtgaatgatttccgtcaggccgtcgccaacgcccgggaagcgggcttcgacctggttgagcttcactctgcgcacggttacctgctgcatcagttcctgtccccgtcttccaaccagcgtaccgaccagtacggcggcagcgttgaaaaccgcgcgcgtctggtgcttgaagtggtggatgctgtctgtaatgagtggagcgcagaccgcattggtattcgtgtctccccgatcggtactttccagaacgtcgacaacggtccgaacgaagaagcagacgcgctgtatctgattgaagagctggcgaaacgcggtatcgcctatctgcacatgtccgagacggacttggcaggcggcaagccttacagtgaagccttccgtcagaaagtgcgcgagcgcttccacggcgtgattatcggggcgggtgcgtatacggcagaaaaagccgaggatttgatcggtaaaggcctgatcgacgccgtggcctttggccgtgactacattgctaacccggatctggttgcccgtttgcagaaaaaagccgaactgaacccgcagcgtcctgaaagcttctatggcggcggcgcggaaggttataccgactaccctacgctgtaataa BBa_R0082_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact BBa_B0034_sequence 1 aaagaggagaaa BBa_E0030_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_K216006_sequence 1 gagtcgttatgtcagctgaaaagctgtttaccccactgaaagtgggtgccgttactgccccaaaccgcgtgtttatggccccacttacccgtctgcgcagcatcgagccgggcgatatcccaacgccattgatgggtgagtattaccgccagcgcgccagcgcgggcctgattatctccgaagccacgcagatttctgctcaggcaaaaggctacgccggtgcaccgggtctgcacagcccggaacagatcgccgcgtggaaaaaaatcaccgcaggcgtgcatgctgaagatggccgtattgcggttcagctgtggcacaccggtcgtatctcacacagcagcatccagcctggcggtcaggcgccggtttctgcctctgccctgaacgccaatacccgcacttccctgcgcgatgaaaacggtaatgcgatccgcgtcgacaccaccacgccacgcgcgctggagctggacgagatcccgggtatcgtgaatgatttccgtcaggccgtcgccaacgcccgggaagcgggcttcgacctggttgagcttcactctgcgcacggttacctgctgcatcagttcctgtccccgtcttccaaccagcgtaccgaccagtacggcggcagcgttgaaaaccgcgcgcgtctggtgcttgaagtggtggatgctgtctgtaatgagtggagcgcagaccgcattggtattcgtgtctccccgatcggtactttccagaacgtcgacaacggtccgaacgaagaagcagacgcgctgtatctgattgaagagctggcgaaacgcggtatcgcctatctgcacatgtccgagacggacttggcaggcggcaagccttacagtgaagccttccgtcagaaagtgcgcgagcgcttccacggcgtgattatcggggcgggtgcgtatacggcagaaaaagccgaggatttgatcggtaaaggcctgatcgacgccgtggcctttggccgtgactacattgctaacccggatctggttgcccgtttgcagaaaaaagccgaactgaacccgcagcgtcctgaaagcttctatggcggcggcgcggaaggttataccgactaccctacgctgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z