BBa_K216013 1 BBa_K216013 TNT receptor TNT-R1 + hybrid signal transduction protein Trz 2009-10-15T11:00:00Z 2015-05-08T01:11:30Z Made by joining BBa_K216004 to BBa_K216002. This consists of the coding sequences for TNT receptor TNT-R1 (made by rational redesign of ribose binding protein by the Hellinga et al, Nature 2003) and the hybrid signal transduction protein Trz (consisting of the extracellular part of chemotaxis receptor Trg fused to the intracellular part of sensor kinase EnvZ, which phosphorylates OmpR, controlling promoters such as PompC; made by Hazelbauer et al). false false _317_ 0 837 163 It's complicated true No special considerations. Note that due to a minor miscalculation, this construct lacks a NotI site in the suffix, but the suffix is otherwise intact; thus it does not fully conform to the standard RFC10, though it can be used in assembly in the usual way. We apologize for this situation and plan to submit a revised version with the missing NotI site, or, alternatively, propose a new standard similar to RFC10 but which does not include the NotI sites in the prefix and suffix. false Edinburgh iGEM 2009 component2218765 1 BBa_K216002 component2218769 1 BBa_K216004 annotation2218765 1 BBa_K216002 range2218765 1 1 851 annotation2218769 1 BBa_K216004 range2218769 1 860 2364 BBa_K216004 1 BBa_K216004 Trz hybrid signal transduction protein 2009-09-24T11:00:00Z 2015-05-08T01:11:30Z Kindly supplied by Prof. G. Hazelbauer, and BioBricked by the University of Edinburgh iGEM team 2009. Trz hybrid signal transduction protein: this was created by fusing the extracellular receptor domain of the chemoreceptor Trg with the intracellular signal transduction domain of 2-component sensor protein EnvZ, which normally phosphorylates response regulator OmpR (see Baumgartner, J.W., Kim, C., Brissette, R.E., Inouye, M., Park, C., and Hazelbauer, G.L. 1994. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of the osmosensor EnvZ. J. Bacteriol. 176, 1157-1163. This part can activate genes attached to an OmpR-responsive promoter such as PompC. To avoid inappropriate activation via EnvZ, it may be necessary to use a chassis in which EnvZ is not present. false false _317_ 0 837 163 It's complicated true No special considerations. false Edinburgh iGEM 2009 annotation2027069 1 STOP range2027069 1 1500 1505 annotation2027067 1 RBS range2027067 1 4 8 annotation2027068 1 START range2027068 1 18 20 BBa_K216002 1 BBa_K216002 TNT Receptor TNT-R1 2009-08-03T11:00:00Z 2015-05-08T01:11:30Z Computationally-derived ligand receptor. Looger, L. L., Dwyer, M. A., Smith, J. J. and Hellinga, H. W. (2003). Computational design of receptor and sensor proteins with novel functions. Nature 423: 185 - 190. TNT.R1 has fair affinity (340nM) for trinitrotoluene (TNT)that is not a naturally occurring compound. TNT.R1 is less thermostable than the wildtype receptor. As such, it is suggested that the receptor be used shortly after expression. TNT receptor 1 (TNT.R1) is a computationally-derived ligand receptor specific for TNT. The ribose-binding pocket of ribose-binding protein, a member of the E. coli periplasmic binding protein (PBP) family, was reconfigured so that the receptor recognizes TNT instead of the wild-type ligand. false false _317_ 0 5058 9 It's complicated true This part is cloned into BioBrick vector pSB1A2. false Edinburgh iGEM 2009 annotation2027071 1 STOP range2027071 1 846 851 annotation2027070 1 START range2027070 1 12 14 annotation2014755 1 RBS range2014755 1 1 6 BBa_K216002_sequence 1 aaggagatatcatgaaagataccattgcattggtagtatccacacttaataatccagcgaacgtatccctgaaagatggcgctcagaaagaagccgacaaactgggctataacctggtcgtcctggactcccagaacaacccggccaaagaactggccaacgtgcaggatctgaccgtgcgcggcaccaaaatcctgctgattagcccgaccgatagcgatgccgttggtaacgccgttaaaatggccaaccaggcgaacattccggtgattaccctgagccgtcaggccaccaaaggcgaagtcgtgtcccatattgccagcgataacgtactgggtggcaaaattgcgggcgattatattgccaaaaaagccggcgagggcgccaaagttatcgaactgcaaggcattgctggcacctctgccgcacgcgaacgtggtgaaggctttcagcaggccgtagccgcacacaaatttaacgttctggcgagccagccggcagatagcgatcgcatcaaaggcctgaacgtgatgcagaatctgctgaccgcacatccggatgtgcaggcggtatttgcgcagaacgatgaaatggcactgggcgcactgcgtgcgctgcaaaccgccggcaaaagcgatgtgatggttgtgggcttcgcgggtaccccggatggcgaaaaagccgttaacgatggcaaactggcggcaaccattgcaagcctgccggatcagattggcgcaaaaggcgtggaaaccgcggataaagtactgaaaggcgaaaaagttcaggcgaaatatccagtagactgcaagcttgtcgtaaaacagaacgggggctcccactggtaataa BBa_K216013_sequence 1 aaggagatatcatgaaagataccattgcattggtagtatccacacttaataatccagcgaacgtatccctgaaagatggcgctcagaaagaagccgacaaactgggctataacctggtcgtcctggactcccagaacaacccggccaaagaactggccaacgtgcaggatctgaccgtgcgcggcaccaaaatcctgctgattagcccgaccgatagcgatgccgttggtaacgccgttaaaatggccaaccaggcgaacattccggtgattaccctgagccgtcaggccaccaaaggcgaagtcgtgtcccatattgccagcgataacgtactgggtggcaaaattgcgggcgattatattgccaaaaaagccggcgagggcgccaaagttatcgaactgcaaggcattgctggcacctctgccgcacgcgaacgtggtgaaggctttcagcaggccgtagccgcacacaaatttaacgttctggcgagccagccggcagatagcgatcgcatcaaaggcctgaacgtgatgcagaatctgctgaccgcacatccggatgtgcaggcggtatttgcgcagaacgatgaaatggcactgggcgcactgcgtgcgctgcaaaccgccggcaaaagcgatgtgatggttgtgggcttcgcgggtaccccggatggcgaaaaagccgttaacgatggcaaactggcggcaaccattgcaagcctgccggatcagattggcgcaaaaggcgtggaaaccgcggataaagtactgaaaggcgaaaaagttcaggcgaaatatccagtagactgcaagcttgtcgtaaaacagaacgggggctcccactggtaataatactagaggacgagagacaacggtaatgaatacaactccctcacagcgattaggttttttgcatcacatcaggttggttccgttatttgcctgcattctaggcggtatcttagttctattcgcattaagttcagccctggctggctatttcctctggcaggccgatcgcgatcagcgtgatgttactgcggagattgagattcggaccgggttagcgaacagttcagattttttgcgttcagcccggatcaatatgattcaggccggggctgcgagtcgtattgcggaaatggaagcaatgaagcgaaatattgcgcaagccgaatcggagattaaacagtcgcagcaaggttatcgtgcttatcagaatcgaccggtgaaaacacctgctgatgaagccctcgacactgaattaaatcaacgctttcaggcttatatcacgggtatgcaacctatgttgaaatatgccaaaaatggcatgtttgaagcgattatcaatcatgaaagtgagcagatccgaccgctggataatgcttataccgatattttgaacaaagccgttaagatacgtagcaccagagccaaccaactggcggaactggcccatcagcgcacccgcctgggtgggatgttcatgattggcgcgtttgtgcttgccctggtcatgacgctgataacatttatggtgctacgtcggatcgtcattcgtccactgcaacatgccgcacaacggattgaaaaaatcgccagtggcgatctgacgatgaatgatgaaccggcgggtcgtaatgaaatcggtcgcttaagtcgtcatttacagcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaataa BBa_K216004_sequence 1 gacgagagacaacggtaatgaatacaactccctcacagcgattaggttttttgcatcacatcaggttggttccgttatttgcctgcattctaggcggtatcttagttctattcgcattaagttcagccctggctggctatttcctctggcaggccgatcgcgatcagcgtgatgttactgcggagattgagattcggaccgggttagcgaacagttcagattttttgcgttcagcccggatcaatatgattcaggccggggctgcgagtcgtattgcggaaatggaagcaatgaagcgaaatattgcgcaagccgaatcggagattaaacagtcgcagcaaggttatcgtgcttatcagaatcgaccggtgaaaacacctgctgatgaagccctcgacactgaattaaatcaacgctttcaggcttatatcacgggtatgcaacctatgttgaaatatgccaaaaatggcatgtttgaagcgattatcaatcatgaaagtgagcagatccgaccgctggataatgcttataccgatattttgaacaaagccgttaagatacgtagcaccagagccaaccaactggcggaactggcccatcagcgcacccgcctgggtgggatgttcatgattggcgcgtttgtgcttgccctggtcatgacgctgataacatttatggtgctacgtcggatcgtcattcgtccactgcaacatgccgcacaacggattgaaaaaatcgccagtggcgatctgacgatgaatgatgaaccggcgggtcgtaatgaaatcggtcgcttaagtcgtcatttacagcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z