LOCUS       BBa_K216014             1060 bp    DNA     linear       19-May-2024
DEFINITION  PyeaR with Renilla luciferase
ACCESSION   BBa_K216014
VERSION     BBa_K216014.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     misc_feature    109..111
                     /label=SacI
     RBS             112..118
                     /label=rbs
     RBS             109..118
                     /label=BBa_J15001
     misc_feature    125..1060
                     /label=Rluc
     CDS             125..1060
                     /label=Rluc
     misc_feature    125..1060
                     /label=BBa_J52008
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagct caaggaggta
      121 ctagatggct tccaaggtgt acgaccccga gcaacgcaaa cgcatgatca ctgggcctca
      181 gtggtgggct cgctgcaagc aaatgaacgt gctggactcc ttcatcaact actatgattc
      241 cgagaagcac gccgagaacg ccgtgatttt tctgcatggt aacgctgcct ccagctacct
      301 gtggaggcac gtcgtgcctc acatcgagcc cgtggctaga tgcatcatcc ctgatctgat
      361 cggaatgggt aagtccggca agagcgggaa tggctcatat cgcctcctgg atcactacaa
      421 gtacctcacc gcttggttcg agctgctgaa ccttccaaag aaaatcatct ttgtgggcca
      481 cgactggggg gcttgtctgg cctttcacta ctcctacgag caccaagaca agatcaaggc
      541 catcgtccat gctgagagtg tcgtggacgt gatcgagtcc tgggacgagt ggcctgacat
      601 cgaggaggat atcgccctga tcaagagcga agagggcgag aaaatggtgc ttgagaataa
      661 cttcttcgtc gagaccatgc tcccaagcaa gatcatgcgg aaactggagc ctgaggagtt
      721 cgctgcctac ctggagccat tcaaggagaa gggcgaggtt agacggccta ccctctcctg
      781 gcctcgcgag atccctctcg ttaagggagg caagcccgac gtcgtccaga ttgtccgcaa
      841 ctacaacgcc taccttcggg ccagcgacga tctgcctaag atgttcatcg agtccgaccc
      901 tgggttcttt tccaacgcta ttgtcgaggg agctaagaag ttccctaaca ccgagttcgt
      961 gaaggtgaag ggcctccact tcagccagga ggacgctcca gatgaaatgg gtaagtacat
     1021 caagagcttc gtggagcgcg tgctgaagaa cgagcagtaa
//