LOCUS       BBa_K2173009            1854 bp    DNA     linear       31-May-2024
DEFINITION  LuxI_LuxR
ACCESSION   BBa_K2173009
VERSION     BBa_K2173009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..78
                     /label=RBS-1\Strong
     RBS             71..75
                     /label=RBS
     misc_feature    64..78
                     /label=BBa_B0030
     RBS             64..78
                     /label=BBa_B0030
     misc_feature    664..695
                     /label=LVA
     CDS             85..663
                     /label=luxI
     misc_feature    85..702
                     /label=BBa_C0061
     misc_feature    703..727
                     /label=Help:Barcodes
     CDS             85..702
                     /label=BBa_C0061
     stem_loop       747..790
                     /label=stem_loop
     misc_feature    736..815
                     /label=BBa_B0010
     terminator      736..815
                     /label=BBa_B0010
     stem_loop       831..850
                     /label=T7 TE
     misc_feature    857..857
                     /label=stop
     polyA_site      851..864
                     /label=polya
     misc_feature    824..864
                     /label=BBa_B0012
     terminator      824..864
                     /label=BBa_B0012
     terminator      736..864
                     /label=BBa_B0015
     promoter        873..907
                     /label=BBa_J23100
     RBS             916..930
                     /label=RBS-1\Strong
     RBS             923..927
                     /label=RBS
     misc_feature    916..930
                     /label=BBa_B0030
     RBS             916..930
                     /label=BBa_B0030
     misc_feature    937..938
                     /label=prefix
     misc_feature    1110..1110
                     /label=T
     misc_feature    1428..1428
                     /label=A
     CDS             937..1686
                     /label=luxR
     misc_feature    937..1692
                     /label=BBa_C0062
     misc_feature    1693..1717
                     /label=Help:Barcodes
     CDS             937..1692
                     /label=BBa_C0062
     stem_loop       1737..1780
                     /label=stem_loop
     misc_feature    1726..1805
                     /label=BBa_B0010
     terminator      1726..1805
                     /label=BBa_B0010
     stem_loop       1821..1840
                     /label=T7 TE
     misc_feature    1847..1847
                     /label=stop
     polyA_site      1841..1854
                     /label=polya
     misc_feature    1814..1854
                     /label=BBa_B0012
     terminator      1814..1854
                     /label=BBa_B0012
     terminator      1726..1854
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagattaaag aggagaaata ctagatgact ataatgataa aaaaatcgga ttttttggca
      121 attccatcgg aggagtataa aggtattcta agtcttcgtt atcaagtgtt taagcaaaga
      181 cttgagtggg acttagttgt agaaaataac cttgaatcag atgagtatga taactcaaat
      241 gcagaatata tttatgcttg tgatgatact gaaaatgtaa gtggatgctg gcgtttatta
      301 cctacaacag gtgattatat gctgaaaagt gtttttcctg aattgcttgg tcaacagagt
      361 gctcccaaag atcctaatat agtcgaatta agtcgttttg ctgtaggtaa aaatagctca
      421 aagataaata actctgctag tgaaattaca atgaaactat ttgaagctat atataaacac
      481 gctgttagtc aaggtattac agaatatgta acagtaacat caacagcaat agagcgattt
      541 ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca aagaaattca tgtattaggt
      601 gatactaaat cggttgtatt gtctatgcct attaatgaac agtttaaaaa agcagtctta
      661 aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt
      721 agatctctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      781 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      841 cgggtgggcc tttctgcgtt tatatactag agttgacggc tagctcagtc ctaggtacag
      901 tgctagctac tagagattaa agaggagaaa tactagatga aaaacataaa tgccgacgac
      961 acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc
     1021 ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct
     1081 cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa
     1141 tattatgatg acgctaattt aataaaatat gatcctatag tagattattc taactccaat
     1201 cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat
     1261 gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg
     1321 gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat
     1381 agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat
     1441 tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa
     1501 gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt
     1561 tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca
     1621 aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt
     1681 aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa
     1741 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1801 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//