BBa_K165002 1 BBa_K165002 Kozak sequence (yeast RBS) 2008-10-25T11:00:00Z 2015-05-08T01:10:55Z - Released HQ 2013 The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region. false false _267_ 0 2512 58 In stock false - false John Szymanski BBa_K2185003 1 BBa_K2185003 Penicillium alpha amylase w/ no promoter 2016-09-04T11:00:00Z 2016-09-15T01:44:50Z Coding sequence - pencilium fungus (sequence from GenBank) This composite part, designed and submitted by the 2016 Broad Run iGEM Team, contains an gene from the fungus Penicilium that codes for an alpha amylase enzyme. This composite part contains the coding sequence, kozak sequence, a mating factor secretion tag, and a terminator. It does not include a promoter. The amylase enzyme can be expressed by cloning this part into a plasmid with a promoter built in (such as the S.cerevisiae yeast vector pAG36). The components of this part are: Kozak Sequence - BBa_K165002 This kozak sequence functions as the ribosome binding site for S.cerevisiae. It is involved in translating the sequence from mRNA to amino acids. Mating Factor Secretion Tag - BBa_K792002 This part is the secretion signal peptide from the S.cerevisiae &#945;-mating factor. This signal peptide directs the secretion of the produced protein, allowing the protein to interact with the environment outside the cell. Fungal Alpha Amylase Coding Sequence - BBa_K2185002 This coding sequence codes for the alpha amylase enzyme from the Pencillium fungus. The enzyme can break alpha bonds linking polysaccharides, thus degrading starch into monosaccharides. ADH1 Terminator - BBa_K392003 This is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. false false _2661_ 27970 27970 9 false Removed Kpn and spe sites. Codon optimized for S.cerevisiae. false Marissa Sumathipala component2483507 1 BBa_K792002 component2483508 1 BBa_K165002 component2483505 1 BBa_K165002 component2483510 1 BBa_K792002 component2483511 1 BBa_K392003 component2483512 1 BBa_K2185002 component2483513 1 BBa_K392003 annotation2483508 1 BBa_K165002 range2483508 1 87 104 annotation2483511 1 BBa_K392003 range2483511 1 175 303 annotation2483507 1 BBa_K792002 range2483507 1 27 80 annotation2483513 1 BBa_K392003 range2483513 1 1583 1711 annotation2483512 1 BBa_K2185002 range2483512 1 87 1574 annotation2483510 1 BBa_K792002 range2483510 1 113 166 annotation2483505 1 BBa_K165002 range2483505 1 1 18 BBa_K392003 1 BBa_K392003 yeast ADH1 terminator 2010-10-09T11:00:00Z 2015-05-08T01:12:20Z Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak). Engineered 'cyc100 promoter' of <i>Saccharomyces cerevisiae</i> with Kozak sequence attached downstream. false false _528_ 0 6220 9 It's complicated true Assembled by 3A method. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K792002 1 MFa1-Secre Secretion tag from yeast &#945;-factor mating pheromone (MF&#945;1) 2012-09-23T11:00:00Z 2015-05-08T01:13:23Z Prepro-a-factorHas a Cleavable Signal Sequence [Water et al 1987] This part is the secretion signal peptide for the yeast &#945;-mating factor. This signal peptide directs the secretion of the produced protein, and therefore allows for the exportation of it. This peptides are cleaved once the protein is in the lumen of the ER. false false _1047_ 0 11811 9 Not in stock false None false Manuel Gim??nez annotation2197873 1 secretion tag range2197873 1 1 54 BBa_K2185002 1 BBa_K2185002 Penicillium alpha amylase coding sequence 2016-09-03T11:00:00Z 2016-09-15T01:31:56Z It comes from the fungus Penicilium. The sequence comes from GenBank. This is a coding sequence for the alpha amylase enzyme from the fungus Pencilium. This coding sequence can be combined with the remaining components of a genetic construct and transformed into a target organism for expression of the enzyme. false false _2661_ 27970 27970 9 Not in stock false Kpn and Spe restriction sites were removed and sequence was optimized for S.cerevisiae. false BRHS iGEM Team annotation2482465 1 secretion tag range2482465 1 27 80 annotation2482466 1 BBa_K792002 range2482466 1 27 80 annotation2482467 1 BBa_K392003 range2482467 1 89 217 annotation2482464 1 BBa_K165002 range2482464 1 1 18 BBa_K165002_sequence 1 cccgccgccaccatggag BBa_K2185003_sequence 1 cccgccgccaccatggagtactagagagattcccatccattttcaccgctgttttgttcgccgcttcttctgctttggcttactagatggttctagctcggcttgcctggcttgccggcctggttagcactgccattgctgcgaccccagcggaatggcgctcgcagtccatctacttcatgctcacagatcgttttgcccggaccgatggttcaactaccgctgcctgcgataccgctgacagaaaatactgtggcggaacctggcagggaatcattgacaagctggactatatccaagggatgggctttacagccatttggatcactccggtgaccggtcaattgagcggggaaaccgcgtacggagatgcctatcacggatactggcagcaggatatctattctctcgattccaactatggaacagcaggcgatctcaaggccctcgctgcggctttgcacaaacgtaacatgtatctcatggtcgatgtcgtagcaaaccatatgggctacaatggcgcaggtgctgatgtggactacaccaaattcaaccccttcaacgaggcaaagtatttccactcctactgcccgatcaccgattacaacgacgacaccatgtcgcaaaactgctggcttggcgataacaaggtctcgctaccggacctgaatacacagagcaaggaggtgcaggatctatggtatgactgggttggatctttggtctccaactactccatcgatggacttcgcgtcgacacagtcaaacatgtccagaaagatttctggcccggctacaacaaagccgcgggcgtctactgcgttggcgaaatccttgatggtgacccagattacacctgtccataccaggaggtaatggacggagtgctcaactacccgatttactacccactcctcaaggccttccagtcgacatcgggaagcatgaccgatctatacaacatgatcaacacggtgaaatcgacctgcaaggactcaacccttcttggaaatttcttggagaaccacgataacccacgttttgcccatgccaccgatgacattgccctcgccaagaacgcagccacatttaccattatggcagatggcattcctattgtctatgcaggacaggagcagcattgcagcggtggcgaggacccggctaatcgcgaggctctgtggctatccggatacaacaccgacagcgagctgtacaagctcattgccaaggccaatggtgctagaaaccaggccattgctaagagtaccaattatactatttaccagaaccacccaatctacaaagacgagagcaccatcgccatgcggaagggcttcgacggcggacagacaatcactgtcctgacgaatctcggtgcagggggtaaagagtattcagtttcgattcctgatactggattcaaggctggtgcgaagttgactgaggttgtgtcctgcacaagtgttactgttggtgatagtggggaggtgtctgttcctatggcgggtggagcgccgaagatcttgctccccacgcctttgctcgagggctcggctctgtgctcatcatagtactagaggcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K2185002_sequence 1 atggttctagctcggcttgcctggcttgccggcctggttagcactgccattgctgcgaccccagcggaatggcgctcgcagtccatctacttcatgctcacagatcgttttgcccggaccgatggttcaactaccgctgcctgcgataccgctgacagaaaatactgtggcggaacctggcagggaatcattgacaagctggactatatccaagggatgggctttacagccatttggatcactccggtgaccggtcaattgagcggggaaaccgcgtacggagatgcctatcacggatactggcagcaggatatctattctctcgattccaactatggaacagcaggcgatctcaaggccctcgctgcggctttgcacaaacgtaacatgtatctcatggtcgatgtcgtagcaaaccatatgggctacaatggcgcaggtgctgatgtggactacaccaaattcaaccccttcaacgaggcaaagtatttccactcctactgcccgatcaccgattacaacgacgacaccatgtcgcaaaactgctggcttggcgataacaaggtctcgctaccggacctgaatacacagagcaaggaggtgcaggatctatggtatgactgggttggatctttggtctccaactactccatcgatggacttcgcgtcgacacagtcaaacatgtccagaaagatttctggcccggctacaacaaagccgcgggcgtctactgcgttggcgaaatccttgatggtgacccagattacacctgtccataccaggaggtaatggacggagtgctcaactacccgatttactacccactcctcaaggccttccagtcgacatcgggaagcatgaccgatctatacaacatgatcaacacggtgaaatcgacctgcaaggactcaacccttcttggaaatttcttggagaaccacgataacccacgttttgcccatgccaccgatgacattgccctcgccaagaacgcagccacatttaccattatggcagatggcattcctattgtctatgcaggacaggagcagcattgcagcggtggcgaggacccggctaatcgcgaggctctgtggctatccggatacaacaccgacagcgagctgtacaagctcattgccaaggccaatggtgctagaaaccaggccattgctaagagtaccaattatactatttaccagaaccacccaatctacaaagacgagagcaccatcgccatgcggaagggcttcgacggcggacagacaatcactgtcctgacgaatctcggtgcagggggtaaagagtattcagtttcgattcctgatactggattcaaggctggtgcgaagttgactgaggttgtgtcctgcacaagtgttactgttggtgatagtggggaggtgtctgttcctatggcgggtggagcgccgaagatcttgctccccacgcctttgctcgagggctcggctctgtgctcatcatag BBa_K792002_sequence 1 agattcccatccattttcaccgctgttttgttcgccgcttcttctgctttggct BBa_K392003_sequence 1 gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z