LOCUS       BBa_K223033             1149 bp    DNA     linear       01-Jun-2024
DEFINITION  trp-operon + RBS + IL-6 + HlyA + TT  Trp-sensitive IL-6 synthesizer
ACCESSION   BBa_K223033
VERSION     BBa_K223033.1  
FEATURES             Location/Qualifiers
     misc_feature    1..140
                     /label=BBa_K223023
     misc_feature    153..156
                     /label=conserved
     RBS             149..160
                     /label=BBa_B0034
     misc_feature    167..1012
                     /label=BBa_K223022
     stem_loop       1032..1075
                     /label=stem_loop
     misc_feature    1021..1100
                     /label=BBa_B0010
     terminator      1021..1100
                     /label=BBa_B0010
     stem_loop       1116..1135
                     /label=T7 TE
     misc_feature    1142..1142
                     /label=stop
     polyA_site      1136..1149
                     /label=polya
     misc_feature    1109..1149
                     /label=BBa_B0012
     terminator      1109..1149
                     /label=BBa_B0012
ORIGIN
        1 ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc
       61 aaatattctg aaatgacctg ttgacaatta atcatcgaac tagttaacta gtacgcaagt
      121 tcacgtaaaa agggtatcca tactagagaa agaggagaaa tactagatgc caacaataac
      181 cactgcacaa attaaaagca cactacagtc tgcaaagcaa tcctctgcaa ataaattgca
      241 ctcagcagga caaagcacga aagatgcatc accggttccg ccgggtgaag actctaaaga
      301 tgttgcggcg ccgcaccgtc agccgctgac ctcttctgaa cgcattgaca aacaaattcg
      361 ttacatcctg gacggcatct ctgccctgcg taaggagacc tgtaacaaaa gcaacatgtg
      421 tgaaagcagc aaagaagcgc tggcagaaaa caacctgaac cttccgaaaa tggctgaaaa
      481 agatggttgc ttccaatctg gcttcaatga agaaacttgc ctggtgaaaa tcatcaccgg
      541 tcttttggag tttgaagtat acctggaata tctgcagaac cgttttgaaa gcagcgagga
      601 acaagcgcgt gctgtgcaga tgagcaccaa agttctgatc cagttcctgc agaaaaaggc
      661 gaaaaatctg gatgcaatca ctaccccgga cccgaccacc aacgctagcc tgctgacgaa
      721 actgcaggcg cagaaccagt ggctgcagga catgaccact catctgattc tgcgcagctt
      781 taaagaattc ctgcagtcta gcctgcgcgc tcttcgtcaa atgaatgcat tagcctatgg
      841 aagtcaggat aatcttaatc cattaattaa tgaaatcagc aaaatcattt cagctgcagg
      901 taattttgat gttaaagagg aaagagctgc agcttcttta ttgcagttgt ccggtaatgc
      961 cagtgatttt tcatatggac ggaactcaat aactttgaca gcatcagcat aatactagag
     1021 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1081 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1141 gcgtttata
//