BBa_K223022 1 BBa_K223022 IL-6 + HlyA 2009-07-05T11:00:00Z 2015-05-08T01:11:31Z IL-6 + HlyA IL-6 + HlyA true false _380_ 0 4533 9 Discontinued false IL-6 + HlyA false Suzanne Bartram BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K223023 1 BBa_K223023 trp-operon (promoter-operator) sequence 2009-07-05T11:00:00Z 2015-05-08T01:11:31Z trp-operon (promoter-operator) sequence trp-operon (promoter-operator) sequence true false _380_ 0 4533 9 Discontinued false trp-operon (promoter-operator) sequence false Suzanne Bartram BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K223033 1 BBa_K223033 trp-operon + RBS + IL-6 + HlyA + TT Trp-sensitive IL-6 synthesizer 2009-07-05T11:00:00Z 2015-05-08T01:11:31Z trp-operon + RBS + IL-6 + HlyA + TT Trp-sensitive IL-6 synthesizer trp-operon + RBS + IL-6 + HlyA + TT Trp-sensitive IL-6 synthesizer true false _380_ 0 4533 9 Discontinued false trp-operon + RBS + IL-6 + HlyA + TT Trp-sensitive IL-6 synthesizer false Suzanne Bartram component2010117 1 BBa_B0010 component2010113 1 BBa_K223023 component2010115 1 BBa_B0034 component2010119 1 BBa_B0012 component2010116 1 BBa_K223022 annotation2010119 1 BBa_B0012 range2010119 1 1109 1149 annotation2010117 1 BBa_B0010 range2010117 1 1021 1100 annotation2010113 1 BBa_K223023 range2010113 1 1 140 annotation2010115 1 BBa_B0034 range2010115 1 149 160 annotation2010116 1 BBa_K223022 range2010116 1 167 1012 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K223023_sequence 1 ctcaaggcgcactcccgttctggataatgttttttgcgccgacatcataacggttctggcaaatattctgaaatgacctgttgacaattaatcatcgaactagttaactagtacgcaagttcacgtaaaaagggtatcca BBa_K223022_sequence 1 atgccaacaataaccactgcacaaattaaaagcacactacagtctgcaaagcaatcctctgcaaataaattgcactcagcaggacaaagcacgaaagatgcatcaccggttccgccgggtgaagactctaaagatgttgcggcgccgcaccgtcagccgctgacctcttctgaacgcattgacaaacaaattcgttacatcctggacggcatctctgccctgcgtaaggagacctgtaacaaaagcaacatgtgtgaaagcagcaaagaagcgctggcagaaaacaacctgaaccttccgaaaatggctgaaaaagatggttgcttccaatctggcttcaatgaagaaacttgcctggtgaaaatcatcaccggtcttttggagtttgaagtatacctggaatatctgcagaaccgttttgaaagcagcgaggaacaagcgcgtgctgtgcagatgagcaccaaagttctgatccagttcctgcagaaaaaggcgaaaaatctggatgcaatcactaccccggacccgaccaccaacgctagcctgctgacgaaactgcaggcgcagaaccagtggctgcaggacatgaccactcatctgattctgcgcagctttaaagaattcctgcagtctagcctgcgcgctcttcgtcaaatgaatgcattagcctatggaagtcaggataatcttaatccattaattaatgaaatcagcaaaatcatttcagctgcaggtaattttgatgttaaagaggaaagagctgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataactttgacagcatcagcataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K223033_sequence 1 ctcaaggcgcactcccgttctggataatgttttttgcgccgacatcataacggttctggcaaatattctgaaatgacctgttgacaattaatcatcgaactagttaactagtacgcaagttcacgtaaaaagggtatccatactagagaaagaggagaaatactagatgccaacaataaccactgcacaaattaaaagcacactacagtctgcaaagcaatcctctgcaaataaattgcactcagcaggacaaagcacgaaagatgcatcaccggttccgccgggtgaagactctaaagatgttgcggcgccgcaccgtcagccgctgacctcttctgaacgcattgacaaacaaattcgttacatcctggacggcatctctgccctgcgtaaggagacctgtaacaaaagcaacatgtgtgaaagcagcaaagaagcgctggcagaaaacaacctgaaccttccgaaaatggctgaaaaagatggttgcttccaatctggcttcaatgaagaaacttgcctggtgaaaatcatcaccggtcttttggagtttgaagtatacctggaatatctgcagaaccgttttgaaagcagcgaggaacaagcgcgtgctgtgcagatgagcaccaaagttctgatccagttcctgcagaaaaaggcgaaaaatctggatgcaatcactaccccggacccgaccaccaacgctagcctgctgacgaaactgcaggcgcagaaccagtggctgcaggacatgaccactcatctgattctgcgcagctttaaagaattcctgcagtctagcctgcgcgctcttcgtcaaatgaatgcattagcctatggaagtcaggataatcttaatccattaattaatgaaatcagcaaaatcatttcagctgcaggtaattttgatgttaaagaggaaagagctgcagcttctttattgcagttgtccggtaatgccagtgatttttcatatggacggaactcaataactttgacagcatcagcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z